In vitro Azole antifungals susceptibility of Candida spp. isolates from HIV-infected patients with periodontitis.

Autor: Lomeli-Martinez SM; Universidad de Guadalajara, Departamento de Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega, Ocotlán, Jalisco, México.; Universidad de Guadalajara, Departamento de Clínicas Odontológicas Integrales, Centro Universitario de Ciencias de la Salud, Guadalajara, Jalisco, México; Universidad de Guadalajara, Departamento de Bienestar y Desarrollo Sustentable, Centro Universitario del Norte, Colotlán, Mexico., González-Hernández LA; Universidad de Guadalajara, Instituto de Investigación en Inmunodeficiencias y VIH (InIVIH), Departamento de Clínicas Médicas, Centro Universitario de Ciencias de la Salud, Guadalajara, Jalisco, México; Antiguo Hospital Civil de Guadalajara 'Fray Antonio Alcalde', Unidad de VIH, Guadalajara, Jalisco, México., Villanueva JFA; Universidad de Guadalajara, Instituto de Investigación en Inmunodeficiencias y VIH (InIVIH), Departamento de Clínicas Médicas, Centro Universitario de Ciencias de la Salud, Guadalajara, Jalisco, México; Antiguo Hospital Civil de Guadalajara 'Fray Antonio Alcalde', Unidad de VIH, Guadalajara, Jalisco, México., Valentín-Goméz E; Universidad de Valencia, Unidad de Investigación GMCA, Departamento de Microbiología y Ecología, Valencia, España; Instituto de Investigación Sanitaria La Fe, Grupo de Investigación en Infección Grave, Valencia, España., Ratkovich-González S; Universidad de Guadalajara, Doctorado en Biología Molecular en Medicina, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Guadalajara, México., Alvarez-Zavala M; Universidad de Guadalajara, Instituto de Investigación en Inmunodeficiencias y VIH (InIVIH), Departamento de Clínicas Médicas, Centro Universitario de Ciencias de la Salud, Guadalajara, Jalisco, México., Sánchez-Reyes K; Universidad de Guadalajara, Instituto de Investigación en Inmunodeficiencias y VIH (InIVIH), Departamento de Clínicas Médicas, Centro Universitario de Ciencias de la Salud, Guadalajara, Jalisco, México., Cabrera-Silva RI; Universidad de Guadalajara, Instituto de Investigación en Inmunodeficiencias y VIH (InIVIH), Departamento de Clínicas Médicas, Centro Universitario de Ciencias de la Salud, Guadalajara, Jalisco, México., Varela-Hernández JJ; Universidad de Guadalajara, Departamento de Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega, Ocotlán, Jalisco, México.. Electronic address: juan.varela@academicos.udg.mx.
Jazyk: angličtina
Zdroj: Journal de mycologie medicale [J Mycol Med] 2022 Aug; Vol. 32 (3), pp. 101294. Date of Electronic Publication: 2022 May 04.
DOI: 10.1016/j.mycmed.2022.101294
Abstrakt: Objective: The objective of the present study was to determine the in vitro Azole antifungals susceptibility of Candida spp. strains isolated from HIV-positive patients with periodontitis.
Methods: Oral examination was performed in 500 HIV-positive patients, of which 228 were included in the study for having periodontitis which and separated in two groups based on their TCD4 + T-cells: (A) n = 110 (≤200 CD4 + ); (B) n = 118 (>200 CD4 + ). Candida spp. were isolated from the subgingival biofilm and crevicular fluid by seeding on CHROMagar plates and confirmed by endpoint PCR and MALDI-TOF. The susceptibility test in vitro for five antifungals was performed using the disc diffusion method.
Results: From the 228 HIV-positive patients with periodontitis, 174 were positive to Candida spp., and 204 isolations were obtained. 138 (67.64%) were C. albicans, and 66 (32.35%) were Candida non-albicans species. The most frequent Candida non-albicans species in order of frequency were C. glabrata with 48 (23.52%), C. tropicalis with 10 (4.9%), C. krusei with 7 (3.43%), and C. dubliniensis with 1 (0.49%). All species presented resistance to any antifungal: 149 to 5-fluorocytosine (73.0%), 149 to fluconazole (73.0%), and 144 to voriconazole (70.7%). Miconazole and econazole presented the highest susceptibility rates with 129 (63.2%) and 130 (63.7%) isolations, respectively.
Conclusion: The Candida spp. involved in periodontitis of HIV-positive patients have a multi-resistant feature. It is necessary to implement recurrent research regarding the antifungal resistance of the Candida spp. that take part in periodontitis pathogenesis to promote an effective treatment in HIV patients.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 SFMM. Published by Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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