Comparison of Mid-turbinate Nasal Swabs, Saliva, and Nasopharyngeal Swabs for SARS-CoV-2 Reverse Transcription-Polymerase Chain Reaction Testing in Pediatric Outpatients.

Autor:	Vos MB; From the Department of Pediatrics (Vos, Cleeton, Heilman, Nayee, Chahroudi, Morris, Lam), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia., Gonzalez MD; From the Department of Pathology (Gonzalez, Jerris, Park, Rogers), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia., Stone C; From the Department of Research Administration (Stone), Children's Healthcare of Atlanta, Atlanta, Georgia., Cleeton R; From the Department of Pediatrics (Vos, Cleeton, Heilman, Nayee, Chahroudi, Morris, Lam), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia., Figueroa J; From the Department of Pediatrics (Figueroa, Nayee, Schoof, Mavigner, Westbrook), Emory University School of Medicine, Atlanta, Georgia., Jerris R; From the Department of Pathology (Gonzalez, Jerris, Park, Rogers), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia., Park SI; From the Department of Pathology (Gonzalez, Jerris, Park, Rogers), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia., Heilman S; From the Department of Pediatrics (Vos, Cleeton, Heilman, Nayee, Chahroudi, Morris, Lam), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia., Nayee R; From the Department of Pediatrics (Vos, Cleeton, Heilman, Nayee, Chahroudi, Morris, Lam), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia.; From the Department of Pediatrics (Figueroa, Nayee, Schoof, Mavigner, Westbrook), Emory University School of Medicine, Atlanta, Georgia., Chahroudi A; From the Department of Pediatrics (Vos, Cleeton, Heilman, Nayee, Chahroudi, Morris, Lam), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia., Schoof N; From the Department of Pediatrics (Figueroa, Nayee, Schoof, Mavigner, Westbrook), Emory University School of Medicine, Atlanta, Georgia., Mavigner M; From the Department of Pediatrics (Figueroa, Nayee, Schoof, Mavigner, Westbrook), Emory University School of Medicine, Atlanta, Georgia., Morris CR; From the Department of Pediatrics (Vos, Cleeton, Heilman, Nayee, Chahroudi, Morris, Lam), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia., Leong T; From the Department of Biostatistics and Bioinformatics, Emory Rollins School of Public Health, Atlanta, Georgia (Leong)., Grindle A; From the Special Care Unit (Grindle), Children's Healthcare of Atlanta, Atlanta, Georgia., Westbrook A; From the Department of Pediatrics (Figueroa, Nayee, Schoof, Mavigner, Westbrook), Emory University School of Medicine, Atlanta, Georgia., Lam W; From the Department of Pediatrics (Vos, Cleeton, Heilman, Nayee, Chahroudi, Morris, Lam), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia.; From the Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta (Lam)., Rogers BB; From the Department of Pathology (Gonzalez, Jerris, Park, Rogers), Children's Healthcare of Atlanta and Emory University School of Medicine, Atlanta, Georgia.
Jazyk:	angličtina
Zdroj:	Archives of pathology & laboratory medicine [Arch Pathol Lab Med] 2022 Sep 01; Vol. 146 (9), pp. 1056-1061.
DOI:	10.5858/arpa.2021-0625-SA
Abstrakt:	Context.—: Diagnostic testing for SARS-CoV-2 in symptomatic and asymptomatic children remains integral to care, particularly for supporting return to and attendance in schools. The concordance of SARS-CoV-2 detection in children, using various specimen types, has not been widely studied. Objective.—: To compare 3 sample types for SARS-CoV-2 polymerase chain reaction (PCR) testing in children, collected and tested at a single facility. Design.—: We prospectively recruited 142 symptomatic and asymptomatic children/young adults into a sample comparison study performed in a single health care system. Each child provided self-collected saliva, and a trained health care provider collected a mid-turbinate nasal swab and nasopharyngeal (NP) swab. Specimens were assayed within 24 hours of collection by using reverse transcription-polymerase chain reaction (RT-PCR) to detect SARS-CoV-2 on a single testing platform. Results.—: Concurrently collected saliva and mid-turbinate swabs had greater than 95% positive agreement with NP swabs when obtained within 10 days of symptom onset. Positive agreement of saliva and mid-turbinate samples collected from children with symptom onset >10 days prior, or without symptoms, was 82% compared to NP swab samples. Cycle threshold (Ct) values for mid-turbinate nasal samples more closely correlated with Ct values from NP samples than from saliva samples. Conclusions.—: These findings suggest that all 3 sample types from children are useful for SARS-CoV-2 diagnostic testing by RT-PCR, and that concordance is greatest when the child has had symptoms of COVID-19 within the past 10 days. This study provides scientific justification for using sample types other than the NP swab for SARS-CoV-2 testing in pediatric populations. Competing Interests: This work was supported by a grant from the National Institutes of Health for Rapid Acceleration of Diagnostics (RADx).
Databáze:	MEDLINE
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