Enzyme histochemistry: a useful tool for examining the spatial distribution of brain ectonucleotidases in (patho)physiological conditions.

Autor:	Grković I; Department of Molecular Biology and Endocrinology, VINČA Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Serbia. istanojevic@vin.bg.ac.rs., Mitrović N; Department of Molecular Biology and Endocrinology, VINČA Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Serbia., Dragić M; Department for General Physiology and Biophysics, Faculty of Biology, University of Belgrade, Belgrade, Serbia., Zarić Kontić M; Department of Molecular Biology and Endocrinology, VINČA Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade, Serbia.
Jazyk:	angličtina
Zdroj:	Histology and histopathology [Histol Histopathol] 2022 Oct; Vol. 37 (10), pp. 919-936. Date of Electronic Publication: 2022 May 16.
DOI:	10.14670/HH-18-471
Abstrakt:	Adenosine 5'-triphosphate (ATP) and other nucleotides and nucleosides, such as adenosine, are versatile signaling molecules involved in many physiological processes and pathological conditions in the nervous system, especially those with an inflammatory component. They can be released from nerve cells, glial cells, and vascular cells into the extracellular space where they exert their function via ionotropic (P2X) or metabotropic (P2Y) receptors. Signaling via extracellular nucleotides and adenosine is regulated by cell-surface located enzymes ectonucleotidases that hydrolyze the nucleotide to the respective nucleoside. This review summarizes a histochemical approach for detection of ectonucleotidase activities in the cryo-sections of brain tissue. The enzyme histochemistry (EHC) might be used as suitable replacement for immunohistochemistry, since it gives information about both localization and activity, thus adding a functional component to a classical histological approach. With this technique, it is possible to visualize spatial distribution and cell-specific localization of ecto-nucleoside triphosphate diphosphohydrolases (NTPDases) and ecto-5'-nucleotidase (eN/CD73) activities during brain development, after different hormonal manipulations, during neurodegeneration, etc. EHC is also suitable for investigation of microglial morphology in different (patho)physiological conditions. Furthermore, the review describes how to quantify EHC results. (©The Author(s) 2022. Open Access. This article is licensed under a Creative Commons CC-BY International License.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu