Holo-Transcriptome Sequences From the Tropical Marine Sponge Cinachyrella alloclada.

Autor:	Desplat Y; Halmos College of Arts and Sciences, Nova Southeastern University, Dania Beach, FL, USA., Warner JF; Department of Biology and Marine Biology, University of North Carolina Wilmington, Wilmington, NC, USA., Lopez JV; Halmos College of Arts and Sciences, Nova Southeastern University, Dania Beach, FL, USA.
Jazyk:	angličtina
Zdroj:	The Journal of heredity [J Hered] 2022 May 16; Vol. 113 (2), pp. 184-187.
DOI:	10.1093/jhered/esab075
Abstrakt:	Marine sponge transcriptomes are underrepresented in current databases. Furthermore, only 2 sponge genomes are available for comparative studies. Here we present the assembled and annotated holo-transcriptome of the common Florida reef sponge from the species Cinachyrella alloclada. After Illumina high-throughput sequencing, the data assembled using Trinity v2.5 confirmed a highly symbiotic organism, with the complexity of high microbial abundance sponges. This dataset is enriched in poly-A selected eukaryotic, rather than microbial transcripts. Overall, 39 813 transcripts with verified sponge sequence homology coded for 8496 unique proteins. The average sequence length was found to be 946 bp with an N50 sequence length of 1290 bp. Overall, the sponge assembly resulted in a GC content of 51.04%, which is within the range of GC bases in a eukaryotic transcriptome. BUSCO scored completeness analysis revealed a completeness of 60.3% and 60.1% based on the Eukaryota and Metazoa databases, respectively. Overall, this study points to an overarching goal of developing the C. alloclada sponge as a useful new experimental model organism. (© The Author(s) 2021. Published by Oxford University Press on behalf of The American Genetic Association. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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