Actin Dynamics as a Multiscale Integrator of Cellular Guidance Cues.

Autor:	Bull AL; Institute for Physical Science and Technology, University of Maryland, College Park, MD, United States.; Department of Physics, University of Maryland, College Park, MD, United States., Campanello L; Institute for Physical Science and Technology, University of Maryland, College Park, MD, United States.; Department of Physics, University of Maryland, College Park, MD, United States., Hourwitz MJ; Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, United States., Yang Q; Institute for Physical Science and Technology, University of Maryland, College Park, MD, United States.; Department of Physics, University of Maryland, College Park, MD, United States., Zhao M; Institute for Regenerative Cures, Department of Ophthalmology and Vision Science, Department of Dermatology, School of Medicine, University of California, Davis, Davis, CA, United States., Fourkas JT; Institute for Physical Science and Technology, University of Maryland, College Park, MD, United States.; Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, United States., Losert W; Institute for Physical Science and Technology, University of Maryland, College Park, MD, United States.; Department of Physics, University of Maryland, College Park, MD, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in cell and developmental biology [Front Cell Dev Biol] 2022 Apr 27; Vol. 10, pp. 873567. Date of Electronic Publication: 2022 Apr 27 (Print Publication: 2022).
DOI:	10.3389/fcell.2022.873567
Abstrakt:	Migrating cells must integrate multiple, competing external guidance cues. However, it is not well understood how cells prioritize among these cues. We investigate external cue integration by monitoring the response of wave-like, actin-polymerization dynamics, the driver of cell motility, to combinations of nanotopographies and electric fields in neutrophil-like cells. The electric fields provide a global guidance cue, and approximate conditions at wound sites in vivo . The nanotopographies have dimensions similar to those of collagen fibers, and act as a local esotactic guidance cue. We find that cells prioritize guidance cues, with electric fields dominating long-term motility by introducing a unidirectional bias in the locations at which actin waves nucleate. That bias competes successfully with the wave guidance provided by the bidirectional nanotopographies. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Bull, Campanello, Hourwitz, Yang, Zhao, Fourkas and Losert.)
Databáze:	MEDLINE
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