Isolation and analyses of lamina propria lymphocytes from mouse intestines.
| Autor: | Kim E; Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA., Tran M; Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.; Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA., Sun Y; Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA., Huh JR; Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.; Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115, USA. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2022 May 06; Vol. 3 (2), pp. 101366. Date of Electronic Publication: 2022 May 06 (Print Publication: 2022). |
| DOI: | 10.1016/j.xpro.2022.101366 |
| Abstrakt: | Investigating intestinal immune responses is critical to understanding local and systemic immunity. However, obtaining resident intestinal immune cells with high cell viability can be challenging. Here, we provide an optimized protocol to isolate lamina propria lymphocytes from the small and large intestines, including lymphocyte activation for cytokine expression analysis and techniques for surface and intracellular antibody staining and flow cytometry. This protocol can be used for isolating and analyzing tissue-resident immune cells from other tissues with specified modifications. For complete details on the use and execution of this protocol, please refer to Kim et al. (2022). Competing Interests: Jun R. Huh is a co-founder of Interon laboratories. He is also a consultant for CJ Bioscience. (© 2022 The Authors.) |
| Databáze: | MEDLINE |
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