The effect of 100-200 nm ZnO and TiO 2 nanoparticles on the in vitro-grown soybean plants.

Autor: Leopold LF; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania. Electronic address: loredana.leopold@usamvcluj.ro., Coman C; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania. Electronic address: cristina.coman@usamvcluj.ro., Clapa D; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania. Electronic address: doina.clapa@usamvcluj.ro., Oprea I; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania. Electronic address: ioana.oprea@usamvcluj.ro., Toma A; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania. Electronic address: tomaalecr@gmail.com., Iancu ȘD; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Faculty of Physics, Babeș-Bolyai University, 1 Kogalniceanu, 400084 Cluj-Napoca, Romania. Electronic address: stefania.iancu@ubbcluj.ro., Barbu-Tudoran L; Electron Microscopy Center, Faculty of Biology and Geology, Babeș,-Bolyai University, 5-7 Clinicilor, 400006 Cluj-Napoca, Romania; National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath, 400293 Cluj-Napoca, Romania. Electronic address: barbu@itim-cj.ro., Suciu M; Electron Microscopy Center, Faculty of Biology and Geology, Babeș,-Bolyai University, 5-7 Clinicilor, 400006 Cluj-Napoca, Romania; National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath, 400293 Cluj-Napoca, Romania. Electronic address: maria.suciu@itim-cj.ro., Ciorîță A; Electron Microscopy Center, Faculty of Biology and Geology, Babeș,-Bolyai University, 5-7 Clinicilor, 400006 Cluj-Napoca, Romania; National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath, 400293 Cluj-Napoca, Romania. Electronic address: alexandra.ciorita@itim-cj.ro., Cadiș AI; Raluca Ripan Institute for Research in Chemistry, Babeș-Bolyai University, 30 Fântânele, 400294 Cluj Napoca, Romania. Electronic address: adrian.cadis@ubbcluj.ro., Mureșan LE; Raluca Ripan Institute for Research in Chemistry, Babeș-Bolyai University, 30 Fântânele, 400294 Cluj Napoca, Romania. Electronic address: laura.muresan@ubbcluj.ro., Perhaița IM; Raluca Ripan Institute for Research in Chemistry, Babeș-Bolyai University, 30 Fântânele, 400294 Cluj Napoca, Romania. Electronic address: ioana.perhaita@ubbcluj.ro., Copolovici L; Institute for Research, Development and Innovation in Technical and Natural Sciences, Aurel Vlaicu University of Arad, 2 Elena Drăgoi, 310330 Arad, Romania; Faculty of Food Engineering, Tourism and Environmental Protection, Development and Innovation in Technical and Natural Sciences, Aurel Vlaicu University of Arad, 2 Elena Drăgoi, 310330 Arad, Romania. Electronic address: lucian.copolovici@uav.ro., Copolovici DM; Institute for Research, Development and Innovation in Technical and Natural Sciences, Aurel Vlaicu University of Arad, 2 Elena Drăgoi, 310330 Arad, Romania; Faculty of Food Engineering, Tourism and Environmental Protection, Development and Innovation in Technical and Natural Sciences, Aurel Vlaicu University of Arad, 2 Elena Drăgoi, 310330 Arad, Romania. Electronic address: dana.copolovici@uav.ro., Copaciu F; Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania. Electronic address: florina.copaciu@usamvcluj.ro., Leopold N; Faculty of Physics, Babeș-Bolyai University, 1 Kogalniceanu, 400084 Cluj-Napoca, Romania. Electronic address: nicolae.leopold@phys.ubbcluj.ro., Vodnar DC; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania. Electronic address: dan.vodnar@usamvcluj.ro., Coman V; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania; Life Sciences Institute, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăștur, 400372 Cluj-Napoca, Romania. Electronic address: vasile.coman@usamvcluj.ro.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2022 Aug; Vol. 216, pp. 112536. Date of Electronic Publication: 2022 May 10.
DOI: 10.1016/j.colsurfb.2022.112536
Abstrakt: Engineered nanomaterials are increasingly used in everyday life applications and, in consequence, significant amounts are being released into the environment. From soil, water, and air they can reach the organelles of edible plants, potentially impacting the food chain and human health. The potential environmental and health impact of these nanoscale materials is of public concern. TiO 2 and ZnO are among the most significant nanomaterials in terms of production amounts. Our study aimed at evaluating the effects of large-scale TiO 2 (~100 nm) and ZnO (~200 nm) nanoparticles on soybean plants grown in vitro. The effect of different concentrations of nanoparticles (10, 100, 1000 mg/L) was evaluated regarding plant morphology and metabolic changes. ZnO nanoparticles showed higher toxicity compared to TiO 2 in the experimental set-up. Overall, elevated levels of chlorophylls and proteins were observed, as well as increased concentrations of ascorbic and dehydroascorbic acids. Also, the decreasing stomatal conductance to water vapor and net CO 2 assimilation rate show higher plant stress levels. In addition, ZnO nanoparticle treatments severely affected plant growth, while TEM analysis revealed ultrastructural changes in chloroplasts and rupture of leaf cell walls. By combining ICP-OES and TEM results, we were able to show that the nanoparticles were metabolized, and their internalization in the soybean plant tissues occurred in ionic forms. This behavior most likely is the main driving force of nanoparticle toxicity.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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