Metabolomic analysis of endometrial cancer by high-resolution magic angle spinning NMR spectroscopy.

Autor: Arda Düz S; Reproductive Sciences & Advanced Bioinformatics Application & Research Center, Inonu University, Malatya, Turkey.; Department of Obstetrics and Gynecology, School of Medicine, Inonu University, 44315, Malatya, Turkey., Mumcu A; Reproductive Sciences & Advanced Bioinformatics Application & Research Center, Inonu University, Malatya, Turkey.; Laboratory of NMR, Scientific and Technological Research Center, Inonu University, Malatya, Turkey., Doğan B; Reproductive Sciences & Advanced Bioinformatics Application & Research Center, Inonu University, Malatya, Turkey.; Department of Biomedical Engineering, School of Engineering, Inonu University, Malatya, Turkey., Yılmaz E; Department of Obstetrics and Gynecology, School of Medicine, Inonu University, 44315, Malatya, Turkey., İnci Çoşkun E; Department of Obstetrics and Gynecology, School of Medicine, Inonu University, 44315, Malatya, Turkey., Sarıdogan E; Reproductive Sciences & Advanced Bioinformatics Application & Research Center, Inonu University, Malatya, Turkey.; Department of Obstetrics and Gynecology, School of Medicine, Inonu University, 44315, Malatya, Turkey., Tuncay G; Reproductive Sciences & Advanced Bioinformatics Application & Research Center, Inonu University, Malatya, Turkey.; Department of Obstetrics and Gynecology, School of Medicine, Inonu University, 44315, Malatya, Turkey., Karaer A; Reproductive Sciences & Advanced Bioinformatics Application & Research Center, Inonu University, Malatya, Turkey. drkaraer@yahoo.com.; Department of Obstetrics and Gynecology, School of Medicine, Inonu University, 44315, Malatya, Turkey. drkaraer@yahoo.com.
Jazyk: angličtina
Zdroj: Archives of gynecology and obstetrics [Arch Gynecol Obstet] 2022 Dec; Vol. 306 (6), pp. 2155-2166. Date of Electronic Publication: 2022 May 14.
DOI: 10.1007/s00404-022-06587-0
Abstrakt: Purpose: To analyze endometrial metabolite profiles between patients with endometrial cancer and controls.
Methods: Seventeen (17) women with endometrium cancer and 18 controls were enrolled in this study. 1 H HR-MAS (High Resolution-Magic Angle Spinning) NMR (Nuclear Magnetic Resonance) spectroscopy data obtained from endometrial tissue samples of patients with endometrial cancer and control group were analyzed with bioinformatics methods.
Results: Principal component analysis (PCA) and the partial least squares discriminant analysis (PLS-DA) score plots obtained with the multivariate statistical analysis of pre-processed spectral data shows a separation between the samples from patients with endometrial cancer and controls. Analysis results suggest that the levels of lactate, glucose, o-phosphoethanolamine, choline, glycerophosphocholine, phosphocholine, leucine, isoleucine, valine, glutamate, glutamine, n-acetyltyrosine, methionine, taurine, alanine, aspartate and phenylalanine are increased in patients with endometrial cancer compared to the controls.
Conclusion: The metabolomics signature of patients with endometrial cancer is different from that of benign endometrial tissue.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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