| Autor: |
Azuraini MJ; School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia., Vigneswari S; Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus 21030, Malaysia., Huong KH; School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia.; Centre of Chemical Biology, Universiti Sains Malaysia, Penang 11900, Malaysia.; School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta, Midoriku, Yokohama 226-8501, Japan., Khairul WM; Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus 21030, Malaysia., H P S AK; School of Industrial Technology, Universiti Sains Malaysia, Penang 11800, Malaysia., Ramakrishna S; Department of Mechanical Engineering, Center for Nanotechnology and Sustainability, National University of Singapore, Singapore 119260, Singapore., Amirul AA; School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia.; Centre of Chemical Biology, Universiti Sains Malaysia, Penang 11900, Malaysia.; Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, Penang 11700, Malaysia. |
| Abstrakt: |
In this study, we described the preparation of sponge-like porous scaffolds that are feasible for medical applications. A porous structure provides a good microenvironment for cell attachment and proliferation. In this study, a biocompatible PHA, poly(3-hydroxybutyrate-co-4-hydroxybutyrate) was blended with gelatine to improve the copolymer's hydrophilicity, while structural porosity was introduced into the scaffold via a combination of solvent casting and freeze-drying techniques. Scanning electron microscopy results revealed that the blended scaffolds exhibited higher porosity when the 4HB compositions of P(3HB- co -4HB) ranged from 27 mol% to 50 mol%, but porosity decreased with a high 4HB monomer composition of 82 mol%. The pore size, water absorption capacity, and cell proliferation assay results showed significant improvement after the final weight of blend scaffolds was reduced by half from the initial 0.79 g to 0.4 g. The pore size of 0.79g-(P27mol%G10) increased three-fold while the water absorption capacity of 0.4g-(P50mol%G10) increased to 325%. Meanwhile, the cell proliferation and attachment of 0.4g-(P50mol%G10) and 0.4g-(P82mol%G7.5) increased as compared to the initial seeding number. Based on the overall data obtained, we can conclude that the introduction of a small amount of gelatine into P(3HB-co-4HB) improved the physical and biological properties of blend scaffolds, and the 0.4g-(P50mol%G10) shows great potential for medical applications considering its unique structure and properties. |
