Cell Aggregate Assembly through Microengineering for Functional Tissue Emergence.

Autor: Eke G; Laboratoire d'Analyse et d'Architecture des Systèmes, Centre National de la Recherche Scientifique (LAAS-CNRS), Université de Toulouse, INSA, UPS, 31400 Toulouse, France., Vaysse L; RESTORE Research Center, Université de Toulouse, INSERM 1301, CNRS 5070, EFS, ENVT, 31100 Toulouse, France., Yao X; Institut de Biologie Valrose, Université Côte d'Azur, 06108 Nice, France., Escudero M; RESTORE Research Center, Université de Toulouse, INSERM 1301, CNRS 5070, EFS, ENVT, 31100 Toulouse, France., Carrière A; RESTORE Research Center, Université de Toulouse, INSERM 1301, CNRS 5070, EFS, ENVT, 31100 Toulouse, France., Trevisiol E; Laboratoire d'Analyse et d'Architecture des Systèmes, Centre National de la Recherche Scientifique (LAAS-CNRS), Université de Toulouse, INSA, UPS, 31400 Toulouse, France., Vieu C; Laboratoire d'Analyse et d'Architecture des Systèmes, Centre National de la Recherche Scientifique (LAAS-CNRS), Université de Toulouse, INSA, UPS, 31400 Toulouse, France., Dani C; Institut de Biologie Valrose, Université Côte d'Azur, 06108 Nice, France., Casteilla L; RESTORE Research Center, Université de Toulouse, INSERM 1301, CNRS 5070, EFS, ENVT, 31100 Toulouse, France., Malaquin L; Laboratoire d'Analyse et d'Architecture des Systèmes, Centre National de la Recherche Scientifique (LAAS-CNRS), Université de Toulouse, INSA, UPS, 31400 Toulouse, France.
Jazyk: angličtina
Zdroj: Cells [Cells] 2022 Apr 20; Vol. 11 (9). Date of Electronic Publication: 2022 Apr 20.
DOI: 10.3390/cells11091394
Abstrakt: Compared to cell suspensions or monolayers, 3D cell aggregates provide cellular interactions organized in space and heterogeneity that better resume the real organization of native tissues. They represent powerful tools to narrow down the gap between in vitro and in vivo models, thanks to their self-evolving capabilities. Recent strategies have demonstrated their potential as building blocks to generate microtissues. Developing specific methodologies capable of organizing these cell aggregates into 3D architectures and environments has become essential to convert them into functional microtissues adapted for regenerative medicine or pharmaceutical screening purposes. Although the techniques for producing individual cell aggregates have been on the market for over a decade, the methodology for engineering functional tissues starting from them is still a young and quickly evolving field of research. In this review, we first present a panorama of emerging cell aggregates microfabrication and assembly technologies. We further discuss the perspectives opened in the establishment of functional tissues with a specific focus on controlled architecture and heterogeneity to favor cell differentiation and proliferation.
Databáze: MEDLINE
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