Metalloendopeptidase ADAM-like Decysin 1 (ADAMDEC1) in Colonic Subepithelial PDGFRα + Cells Is a New Marker for Inflammatory Bowel Disease.

Autor: Ha SE; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Jorgensen BG; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Wei L; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Jin B; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Kim MS; Department of Physiology, Wonkwang Digestive Disease Research Institute and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University, Iksan 54538, Korea., Poudrier SM; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Singh R; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Bartlett A; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Zogg H; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Kim S; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Baek G; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Kurahashi M; Department of Internal Medicine, Division of Gastroenterology and Hepatology, University of Iowa, Iowa City, IA 52242, USA., Lee MY; Department of Physiology, Wonkwang Digestive Disease Research Institute and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University, Iksan 54538, Korea., Kim YS; Department of Physiology, Wonkwang Digestive Disease Research Institute and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University, Iksan 54538, Korea., Choi SC; Department of gastroenterology, Wonkwang Digestive Disease Research Institute and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University, Iksan 54538, Korea., Sasse KC; Sasse Surgical Associates, Reno, NV 89502, USA., Rubin SJS; Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, CA 94305, USA., Gottfried-Blackmore A; Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, CA 94305, USA., Becker L; Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, CA 94305, USA., Habtezion A; Gastroenterology and Hepatology, Stanford University School of Medicine, Stanford, CA 94305, USA., Sanders KM; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA., Ro S; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 Apr 30; Vol. 23 (9). Date of Electronic Publication: 2022 Apr 30.
DOI: 10.3390/ijms23095007
Abstrakt: Metalloendopeptidase ADAM-Like Decysin 1 (ADAMDEC1) is an anti-inflammatory peptidase that is almost exclusively expressed in the gastrointestinal (GI) tract. We have recently found abundant and selective expression of Adamdec1 in colonic mucosal PDGFRα + cells. However, the cellular origin for this gene expression is controversial as it is also known to be expressed in intestinal macrophages. We found that Adamdec1 mRNAs were selectively expressed in colonic mucosal subepithelial PDGFRα + cells. ADAMDEC1 protein was mainly released from PDGFRα + cells and accumulated in the mucosal layer lamina propria space near the epithelial basement membrane. PDGFRα + cells significantly overexpressed Adamdec1 mRNAs and protein in DSS-induced colitis mice. Adamdec1 was predominantly expressed in CD45 - PDGFRα + cells in DSS-induced colitis mice, with only minimal expression in CD45 + CD64 + macrophages. Additionally, overexpression of both ADAMDEC1 mRNA and protein was consistently observed in PDGFRα + cells, but not in CD64 + macrophages found in human colonic mucosal tissue affected by Crohn's disease. In summary, PDGFRα + cells selectively express ADAMDEC1, which is localized to the colon mucosa layer. ADAMDEC1 expression significantly increases in DSS-induced colitis affected mice and Crohn's disease affected human tissue, suggesting that this gene can serve as a diagnostic and/or therapeutic target for intestinal inflammation and Crohn's disease.
Databáze: MEDLINE
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