Capsule-Targeting Depolymerases Derived from Acinetobacter baumannii Prophage Regions.

Autor: Drobiazko AY; Moscow Institute of Physics and Technology (National Research University), 141700 Dolgoprudny, Russia.; Center of Life Sciences, Skolkovo Institute of Science and Technology, 121205 Skolkovo, Russia., Kasimova AA; N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia., Evseev PV; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia., Shneider MM; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia., Klimuk EI; Institute of Molecular Genetics of National Research Centre «Kurchatov Institute», 123182 Moscow, Russia., Shashkov AS; N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia., Dmitrenok AS; N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia., Chizhov AO; N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia., Slukin PV; State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia., Skryabin YP; State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia., Volozhantsev NV; State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia., Miroshnikov KA; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia., Knirel YA; N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia., Popova AV; State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 Apr 29; Vol. 23 (9). Date of Electronic Publication: 2022 Apr 29.
DOI: 10.3390/ijms23094971
Abstrakt: In this study, several different depolymerases encoded in the prophage regions of Acinetobacter baumannii genomes have been bioinformatically predicted and recombinantly produced. The identified depolymerases possessed multi-domain structures and were identical or closely homologous to various proteins encoded in other A. baumannii genomes. This means that prophage-derived depolymerases are widespread, and different bacterial genomes can be the source of proteins with polysaccharide-degrading activities. For two depolymerases, the specificity to capsular polysaccharides (CPSs) of A. baumannii belonging to K1 and K92 capsular types (K types) was determined. The data obtained showed that the prophage-derived depolymerases were glycosidases that cleaved the A. baumannii CPSs by the hydrolytic mechanism to yield monomers and oligomers of the K units. The recombinant proteins with established enzymatic activity significantly reduced the mortality of Galleria mellonella larvae infected with A. baumannii of K1 and K92 capsular types. Therefore, these enzymes can be considered as suitable candidates for the development of new antibacterials against corresponding A. baumannii K types.
Databáze: MEDLINE
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