Targeting Cell Death Mechanism Specifically in Triple Negative Breast Cancer Cell Lines.

Autor: Pruteanu LL; Department of Chemistry, Centre for Molecular Science Informatics, University of Cambridge, Cambridge CB2 1EW, UK.; MedFuture Research Center for Advanced Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400377 Cluj-Napoca, Romania.; Department of Chemistry and Biology, North University Center at Baia Mare, Technical University of Cluj-Napoca, 4800 Baia Mare, Romania., Braicu C; Research Center for Functional Genomics, Biomedicine and Translational Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania., Módos D; Department of Chemistry, Centre for Molecular Science Informatics, University of Cambridge, Cambridge CB2 1EW, UK., Jurj MA; Research Center for Functional Genomics, Biomedicine and Translational Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania., Raduly LZ; Research Center for Functional Genomics, Biomedicine and Translational Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania., Zănoagă O; Research Center for Functional Genomics, Biomedicine and Translational Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania., Magdo L; Research Center for Functional Genomics, Biomedicine and Translational Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania., Cojocneanu R; Research Center for Functional Genomics, Biomedicine and Translational Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania., Paşca S; Research Center for Functional Genomics, Biomedicine and Translational Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania., Moldovan C; MedFuture Research Center for Advanced Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400377 Cluj-Napoca, Romania.; Department of Pharmaceutical Physics-Biophysics, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania., Moldovan AI; MedFuture Research Center for Advanced Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400377 Cluj-Napoca, Romania.; Department of Pharmaceutical Physics-Biophysics, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania., Ţigu AB; MedFuture Research Center for Advanced Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400377 Cluj-Napoca, Romania., Gurzău E; Environmental Health Center, 400240 Cluj-Napoca, Romania., Jäntschi L; Institute for Doctoral Studies, Babeş-Bolyai University, 400084 Cluj-Napoca, Romania.; Department of Physics and Chemistry, Technical University of Cluj-Napoca, 400641 Cluj-Napoca, Romania., Bender A; Department of Chemistry, Centre for Molecular Science Informatics, University of Cambridge, Cambridge CB2 1EW, UK., Berindan-Neagoe I; Research Center for Functional Genomics, Biomedicine and Translational Medicine, 'Iuliu Hațieganu' University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2022 Apr 26; Vol. 23 (9). Date of Electronic Publication: 2022 Apr 26.
DOI: 10.3390/ijms23094784
Abstrakt: Triple negative breast cancer (TNBC) is currently associated with a lack of treatment options. Arsenic derivatives have shown antitumoral activity both in vitro and in vivo; however, their mode of action is not completely understood. In this work we evaluate the response to arsenate of the double positive MCF-7 breast cancer cell line as well as of two different TNBC cell lines, Hs578T and MDA-MB-231. Multimodal experiments were conducted to this end, using functional assays and microarrays. Arsenate was found to induce cytoskeletal alteration, autophagy and apoptosis in TNBC cells, and moderate effects in MCF-7 cells. Gene expression analysis showed that the TNBC cell lines' response to arsenate was more prominent in the G2M checkpoint, autophagy and apoptosis compared to the Human Mammary Epithelial Cells (HMEC) and MCF-7 cell lines. We confirmed the downregulation of anti-apoptotic genes (MCL1, BCL2, TGFβ1 and CCND1) by qRT-PCR, and on the protein level, for TGFβ2, by ELISA. Insight into the mode of action of arsenate in TNBC cell lines it is provided, and we concluded that TNBC and non-TNBC cell lines reacted differently to arsenate treatment in this particular experimental setup. We suggest the future research of arsenate as a treatment strategy against TNBC.
Databáze: MEDLINE
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