Determination of anthraquinones in Rhamnus purshiana using high-performance liquid chromatography coupled to diode array detector and simple ultraviolet spectroscopic analysis.

Autor: Migues VH; Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil., Mauricio J; Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil., Gomes AF; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista, Brazil., David JP; Instituto de Química, Universidade Federal da Bahia, Salvador, Brazil.
Jazyk: angličtina
Zdroj: Journal of separation science [J Sep Sci] 2022 Jul; Vol. 45 (14), pp. 2478-2487. Date of Electronic Publication: 2022 May 18.
DOI: 10.1002/jssc.202200148
Abstrakt: A new method based on Ultraviolet spectrophotometry was developed and compared with that based on high-performance liquid chromatography for the determination and quantification of anthraquinones in the extracts of Rhamnus purshiana bark. A validated quantitative analysis of cascaroside A, cascaroside B, emodin, and aloe-emodin in these herbal products has been previously performed using high-performance liquid chromatography coupled with a diode array detector. In the high-performance liquid chromatography analysis, all the anthraquinones showed satisfactory regression (r 2 > 0.98) within the test ranges, and the recovery was in the range of 94-117%. The limits of detection and quantification were 0.008-0.010 and 0.029-0.035 μg/mL, respectively. Hierarchical cluster analysis and principal component analysis showed differences in the anthraquinones determined from herbal samples. Subsequently, a simple and low-cost ultraviolet spectrophotometric methodology for the quantitative analysis of the same compounds in the extracts was applied, and all the contents were determined. A paired t-test confirmed that there were no significant differences between the two methods. Our results revealed that the developed method is simple and provides the ability to discriminate and control the quality of anthraquinones in herbal products.
(© 2022 Wiley-VCH GmbH.)
Databáze: MEDLINE
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