Chemiluminescent Probes with Long-Lasting High Brightness for In Vivo Imaging of Neutrophils.

Autor: Huang J; School of Chemical and Biomedical Engineering, 70 Nanyang Drive, Singapore, 637457, Singapore., Cheng P; School of Chemical and Biomedical Engineering, 70 Nanyang Drive, Singapore, 637457, Singapore., Xu C; School of Chemical and Biomedical Engineering, 70 Nanyang Drive, Singapore, 637457, Singapore., Liew SS; School of Chemical and Biomedical Engineering, 70 Nanyang Drive, Singapore, 637457, Singapore., He S; School of Chemical and Biomedical Engineering, 70 Nanyang Drive, Singapore, 637457, Singapore., Zhang Y; National Engineering Research Centre for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P. R. China., Pu K; School of Chemical and Biomedical Engineering, 70 Nanyang Drive, Singapore, 637457, Singapore.; School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, 637371, Singapore.; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 636921, Singapore.
Jazyk: angličtina
Zdroj: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2022 Jul 25; Vol. 61 (30), pp. e202203235. Date of Electronic Publication: 2022 Jun 01.
DOI: 10.1002/anie.202203235
Abstrakt: Real-time optical imaging of immune cells can contribute to understanding their pathophysiological roles, which still remains challenging. Current sensitive chemiluminophores have issues of short half-lives and low brightness, limiting their ability for in vivo longitudinal monitoring of immunological processes. To tackle these issues, we report benzoazole-phenoxyl-dioxetane (BAPD)-based chemiluminophores with intramolecular hydrogen bonding for in vivo imaging of neutrophils. Compared with the classical counterpart, chemiluminescence half-lives and brightness of BAPDs in the aqueous solution are increased by ∼ 33- and 8.2-fold, respectively. Based on the BAPD scaffold, a neutrophil elastase-responsive chemiluminescent probe is developed for real-time imaging of neutrophils in peritonitis and psoriasis mouse models. Our study provides an intramolecular hydrogen bonding molecular design for improving the performance of chemiluminophores in advanced imaging applications.
(© 2022 Wiley-VCH GmbH.)
Databáze: MEDLINE
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