Transcriptome profiling of subepithelial PDGFRα cells in colonic mucosa reveals several cell-selective markers.

Autor: Ha SE; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Jin B; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Jorgensen BG; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Zogg H; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Wei L; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Singh R; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Park C; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Kurahashi M; Department of Internal Medicine, Division of Gastroenterology and Hepatology, University of Lowa, Lowa City, Lowa, United States of America., Kim S; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Baek G; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Poudrier SM; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Lee MY; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America.; Department of Physiology, Wonkwang Digestive Disease Research Institute and Institute of Wonkwang Medical Science, School of Medicine, Wonkwang University, Iksan, Chonbuk, Korea., Sanders KM; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America., Ro S; Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, Nevada, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2022 May 13; Vol. 17 (5), pp. e0261743. Date of Electronic Publication: 2022 May 13 (Print Publication: 2022).
DOI: 10.1371/journal.pone.0261743
Abstrakt: Subepithelial platelet-derived growth factor receptor alpha (PDGFRα)+ cells found in the colonic mucosal tissue come in close contact with epithelial cells, immune cells, neurons, capillaries, and lymphatic networks. Mucosal subepithelial PDGFRα+ cells (MuPαC) are important regulators in various intestinal diseases including fibrosis and inflammation. However, the transcriptome of MuPαC has not yet been elucidated. Using Pdgfra-eGFP mice and flow cytometry, we isolated colonic MuPαC and obtained their transcriptome data. In analyzing the transcriptome, we identified three novel, and selectively expressed, markers (Adamdec1, Fin1, and Col6a4) found in MuPαC. In addition, we identified a unique set of MuPαC-enriched genetic signatures including groups of growth factors, transcription factors, gap junction proteins, extracellular proteins, receptors, cytokines, protein kinases, phosphatases, and peptidases. These selective groups of genetic signatures are linked to the unique cellular identity and function of MuPαC. Furthermore, we have added this MuPαC transcriptome data to our Smooth Muscle Genome Browser that contains the transcriptome data of jejunal and colonic smooth muscle cells (SMC), interstitial cells of Cajal (ICC), and smooth muscle resident PDGFRα+ cells: (https://med.unr.edu/physio/transcriptome). This online resource provides a comprehensive reference of all currently known genetic transcripts expressed in primary MuPαC in the colon along with smooth muscle resident PDGFRα cells, SMC, and ICC in the murine colon and jejunum.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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