Bee gomogenat rescues lymphoid organs from degeneration by regulating the crosstalk between apoptosis and autophagy in streptozotocin-induced diabetic mice.

Autor: Badr G; Zoology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt. badr73@yahoo.com.; Laboratory of Immunology, Zoology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt. badr73@yahoo.com., Sayed LH; Zoology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt.; Laboratory of Immunology, Zoology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt., Omar HEM; Zoology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt., ِAbd Elghaffar SK; Pathology and clinical pathology Department, Faculty of Veterinary Medicine, Assiut University, Assiut, 71516, Egypt., Menshawy MM; Department of Biology, Misr University of Science and Technology, 6th October City, Egypt.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2022 Sep; Vol. 29 (45), pp. 68990-69007. Date of Electronic Publication: 2022 May 13.
DOI: 10.1007/s11356-022-20457-x
Abstrakt: Diabetes mellitus (DM) is a metabolic disorder that causes severe complications in several tissues due to redox imbalances, which in turn cause defective angiogenesis in response to ischemia and activate a number of proinflammatory pathways. Our study aimed to investigate the effect of bee gomogenat (BG) dietary supplementation on the architecture of immune organs in a streptozotocin (STZ)-induced type 1 diabetes (T1D) mouse model. Three animal groups were used: the control non-diabetic, diabetic, and BG-treated diabetic groups. STZ-induced diabetes was associated with increased levels of blood glucose, ROS, and IL-6 and decreased levels of IL-2, IL-7, IL-4, and GSH. Moreover, diabetic mice showed alterations in the expression of autophagy markers (LC3, Beclin-1, and P62) and apoptosis markers (Bcl-2 and Bax) in the thymus, spleen, and lymph nodes. Most importantly, the phosphorylation level of AKT (a promoter of cell survival) was significantly decreased, but the expression levels of MCP-1 and HSP-70 (markers of inflammation) were significantly increased in the spleen and lymph nodes in diabetic mice compared to control animals. Interestingly, oral supplementation with BG restored the levels of blood glucose, ROS, IL-6, IL-2, IL-4, IL-7, and GSH in diabetic mice. Treatment with BG significantly abrogated apoptosis and autophagy in lymphoid organs in diabetic mice by restoring the expression levels of LC3, Beclin-1, P62, Bcl-2, and Bax; decreasing inflammatory signals by downregulating the expression of MCP-1 and HSP-70; and promoting cell survival by enhancing the phosphorylation of AKT. Our data were the first to reveal the therapeutic potential of BG on the architecture of lymphoid organs and enhancing the immune system during T1D.
(© 2022. The Author(s).)
Databáze: MEDLINE
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