Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins.
Autor: | Yuge S; Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan., Nishiyama K; International Research Center for Medical Sciences, Kumamoto University, Kumamoto City, Kumamoto, 860-0811, Japan. koichi_nishiyama@med.miyazaki-u.ac.jp.; Laboratory of Vascular and Cellular Dynamics, Department of Medical Sciences, University of Miyazaki, Miyazaki City, Miyazaki, 889-1962, Japan. koichi_nishiyama@med.miyazaki-u.ac.jp., Arima Y; International Research Center for Medical Sciences, Kumamoto University, Kumamoto City, Kumamoto, 860-0811, Japan.; Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, Kumamoto City, Kumamoto, Japan., Hanada Y; International Research Center for Medical Sciences, Kumamoto University, Kumamoto City, Kumamoto, 860-0811, Japan.; Department of Cardiology, Graduate School of Medicine, Nagoya University, Nagoya City, Aichi, 466-8550, Japan., Oguri-Nakamura E; Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan., Hanada S; International Research Center for Medical Sciences, Kumamoto University, Kumamoto City, Kumamoto, 860-0811, Japan., Ishii T; Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan., Wakayama Y; Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, 565-8565, Japan., Hasegawa U; Department of Materials Science and Engineering, Pennsylvania State University, University Park, PA, 16802, USA., Tsujita K; Biosignal Research Center, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.; Division of Membrane Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan., Yokokawa R; Department of Micro Engineering, Graduate School of Engineering, Kyoto University, Kyoto, 615-8540, Japan., Miura T; Department of Anatomy and Cell Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka City, Fukuoka, 812-8582, Japan., Itoh T; Biosignal Research Center, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.; Division of Membrane Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan., Tsujita K; Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, Kumamoto City, Kumamoto, Japan., Mochizuki N; Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, 565-8565, Japan., Fukuhara S; Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan. s-fukuhara@nms.ac.jp. |
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Jazyk: | angličtina |
Zdroj: | Nature communications [Nat Commun] 2022 May 12; Vol. 13 (1), pp. 2594. Date of Electronic Publication: 2022 May 12. |
DOI: | 10.1038/s41467-022-30197-8 |
Abstrakt: | Angiogenesis is regulated in coordinated fashion by chemical and mechanical cues acting on endothelial cells (ECs). However, the mechanobiological mechanisms of angiogenesis remain unknown. Herein, we demonstrate a crucial role of blood flow-driven intraluminal pressure (IP) in regulating wound angiogenesis. During wound angiogenesis, blood flow-driven IP loading inhibits elongation of injured blood vessels located at sites upstream from blood flow, while downstream injured vessels actively elongate. In downstream injured vessels, F-BAR proteins, TOCA1 and CIP4, localize at leading edge of ECs to promote N-WASP-dependent Arp2/3 complex-mediated actin polymerization and front-rear polarization for vessel elongation. In contrast, IP loading expands upstream injured vessels and stretches ECs, preventing leading edge localization of TOCA1 and CIP4 to inhibit directed EC migration and vessel elongation. These data indicate that the TOCA family of F-BAR proteins are key actin regulatory proteins required for directed EC migration and sense mechanical cell stretching to regulate wound angiogenesis. (© 2022. The Author(s).) |
Databáze: | MEDLINE |
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