Nitric oxide contributes to liver inflammation and parasitic burden control in Ascaris suum infection.

Autor: Oliveira FMS; Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Brazil., Kraemer L; Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Brazil., Cavalcanti da Silva C; Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Brazil., Nogueira DS; Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Brazil., Gazzinelli-Guimarães AC; Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Brazil., Gazzinelli-Guimarães PH; Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Brazil., Barbosa FS; Centro Universitário de Formiga (UNIFOR-MG), Brazil., Resende NM; Laboratory of Sciences Applied to Immunology and Biochemistry of Health and Sport. Department of of Physical Education, Universidade Federal de Lavras, Brazil., Caliari MV; Laboratory of Protozooses, Department of Pathology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Brazil., Gaze ST; Cellular and Molecular Immunology Group, René Rachou Institute, Oswaldo Cruz Foundation - FIOCRUZ, Brazil., Bartholomeu DC; Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Brazil., Fujiwara RT; Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Brazil., Bueno LL; Laboratory of Immunology and Genomics of Parasites, Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Brazil. Electronic address: llbueno@icb.ufmg.br.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2022 Jul; Vol. 238, pp. 108267. Date of Electronic Publication: 2022 May 10.
DOI: 10.1016/j.exppara.2022.108267
Abstrakt: Background: Human ascariasis is one of the most prevalent neglected tropical diseases worldwide. The immune response during human ascariasis is characterized by Th2 polarization and a mixed Th2/Th17 response during the pathogenesis of experimental larval ascariasis. Cytokines and other pro-inflammatory mediators, such as nitric oxide (NO), are involved in helminthic infections. However, the role of NO in ascariasis remains unclear.
Objectives: Given the importance of NO in inflammation, we aimed to determine the immunological and histopathological alterations in the livers of C57BL/6 iNOS -/- mice during A. suum infection.
Methods: In this study, parasitic load was evaluated in the livers of wild type C57BL/6 and C57BL/6 iNOS -/- mice infected with A. suum. Histopathological and morphometric analyses and analysis of serum cytokines via Cytometric Bead Array were performed, and the activity of eosinophil peroxidase and myeloperoxidase of neutrophils in the tissues were determined.
Results: The results showed that NO is important for controlling parasitic load during infection by A. suum. C57BL/6iNOS -/- mice showed reduced inflammatory processes and less tissue damage during liver larval migration of A. suum, which is associated with a reduction in serum levels of pro-inflammatory cytokines.
Conclusions: We demonstrated that NO is a crucial inflammatory molecule during Ascaris sp. infection and controls the establishment of the parasite and the development of the host immune response in the liver.
(Copyright © 2022. Published by Elsevier Inc.)
Databáze: MEDLINE
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