| Autor: |
Gao Y; State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092, PR China., Wei Y; Shanghai Engineering Research Center of Hadal Science and Technology, College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, PR China., Wang P; State Key Laboratory of Marine Geology, Tongji University, Shanghai 200092, PR China. |
| Abstrakt: |
A Gram-stain-negative bacterial strain, designated as E165 T , was isolated from a tidal flat sediment of the East China Sea. Strain E165 T grew optimally at pH 6, at 32 °C and with 1-2 % (w/v) NaCl. The 16S rRNA gene sequence similarity results revealed that strain E165 T was most closely related to Marinomonas rhizomae IVIA-Po-145 T , Marinomonas polaris CK13 T , Marinomonas foliarum IVIA-Po-155 T , Marinomonas hwangdonensis HDW-15 T , Marinomonas pontica 46-16 T , Marinomonas mangrovi B20-1 T and Marinomonas shanghaiensis DSL-35 T with values of 97.0-98.5 %. The digital DNA-DNA hybridization and average nucleotide identity values between strain E165 T and the reference strains were 21.9-34.3 % and 77.6-87.3 %, respectively. The DNA G+C content of the isolate was 42.9 mol%. Strain E165 T contained Q-8 as the sole ubiquinone and C 16 : 0 , summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ) and summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) as the major fatty acids. The major polar lipids of strain E165 T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminolipid and aminophospholipid. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, a novel species, Marinomonas lutimaris sp. nov., is proposed with E165 T (=MCCC 1K06241 T =KCTC 82809 T ) as the type strain. |
