Exploring the Therapeutic Potentials of Exopolysaccharides Derived From Lactic Acid Bacteria and Bifidobacteria: Antioxidant, Antitumor, and Periodontal Regeneration.

Autor: Khalil MA; Biology Department, College of Science, Taif University, Taif, Saudi Arabia.; Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta, Egypt., Sonbol FI; Pharmaceutical Microbiology Department, Faculty of Pharmacy, Tanta University, Tanta, Egypt., Al-Madboly LA; Pharmaceutical Microbiology Department, Faculty of Pharmacy, Tanta University, Tanta, Egypt., Aboshady TA; Periodontology, Oral Medicine, Diagnosis and Radiology Department, Faculty of Dentistry, Tanta University, Tanta, Egypt.; Oral and Maxillofacial Surgery and Diagnostic Sciences, Faculty of Dentistry, Taif University, Taif, Saudi Arabia., Alqurashi AS; Biology Department, College of Science, Taif University, Taif, Saudi Arabia., Ali SS; Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta, Egypt.; Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, China.
Jazyk: angličtina
Zdroj: Frontiers in microbiology [Front Microbiol] 2022 Apr 25; Vol. 13, pp. 803688. Date of Electronic Publication: 2022 Apr 25 (Print Publication: 2022).
DOI: 10.3389/fmicb.2022.803688
Abstrakt: The metabolites of lactic acid bacteria (LAB) and bifidobacteria (Bb) have recently received a lot of attention due to their ability to protect interactions in blood and tissues, as well as their biodegradability and biocompatibility in human tissue. Exopolysaccharides (EPS) derived from bacteria have a long history of use in therapeutic and other industrial applications with no adverse effects. In this regard, EPSs were isolated and characterized from LAB and Bb culture supernatants to determine their antioxidant, antitumor, and periodontal regeneration properties. The antioxidant capacity of the EPSs varied with concentration (0.625-20 mg/ml). The highest antioxidant activity was found in LAB: Streptococcus thermophiles DSM 24731-EPS 1 , Lactobacillus delbrueckii ssp. bulgaricus DSM 20081 T -EPS 5 , Limosilactobacillus fermentum DSM 20049-EPS 6 , and Bb; Bifidobacterium longum ssp. longum DSM 200707-EPS 10 . Human breast cancer cells (MCF7), human colon cancer cells (CaCo2), human liver cancer cells (HepG2), and human embryonic kidney 293 (HEK 293) cells were used as controls to assess the antitumor properties of the selected EPSs. According to the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay, EPS 5 had the highest cytotoxicity against MCF7, CaCo2, and HepG2, with IC 50 values of 7.91, 10.69, and 9.12 mg/ml, respectively. Lactate dehydrogenase (LDH) activity was significantly higher in cell lines treated with EPS 5 -IC 50 values compared to other EPSs-IC 50 values ( p < 0.05). Real time (RT)-PCR results showed that EPS 5 treatment increased Bax , Caspase 8 , Caspase 3 , and p53 gene expression. The expression of the BCL2 , MCL1 , and Vimentin genes, on the other hand, was reduced. The MTT test was used to examine the effect of EPS 5 on the viability of human periodontal ligament fibroblast cells (hPDLFCs), and it was discovered that EPS 5 increased hPDLFC viability. According to high-performance liquid chromatography (HPLC) analysis, galactose made up 12.5% of EPS 5 . The findings of this study pave the way for the use of EPS, which hold great promise for a variety of therapeutic purposes such as antioxidant, antitumor, and periodontal regeneration.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Khalil, Sonbol, Al-Madboly, Aboshady, Alqurashi and Ali.)
Databáze: MEDLINE
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