Changes in the urine volatile metabolome throughout growth of transplanted hepatocarcinoma.

Autor: Kochevalina MY; Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia., Bukharina AB; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia., Trunov VG; Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia., Pento AV; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia., Morozova OV; N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, Russia., Kogun' GA; Cynological Division of Aviation Security Service, Aeroflot, Russian Airlines, Moscow, Russia., Simanovsky YO; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia., Nikiforov SM; Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, Russia., Rodionova EI; Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia. a.rodionova@gmail.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 May 11; Vol. 12 (1), pp. 7774. Date of Electronic Publication: 2022 May 11.
DOI: 10.1038/s41598-022-11818-0
Abstrakt: Trained detection dogs distinguish between urine samples from healthy organisms and organisms with malignant tumors, suggesting that the volatile urine metabolome contains information about tumor progression. The aim of this study was to determine whether the stage of tumor growth affects the chemical differences in the urine of mice and to what extent the "olfactory image of disease" perceived by dogs coincides with the "image of disease" recorded by the mass spectrometer. We used a novel laser ionization mass spectrometry method and propose a mass spectrometric analysis without detailed interpretation of the spectrum of volatile metabolomes in urine. The mass spectrometer we use works without sample preparation and registers volatile organic compounds in air at room temperature without changing the pH of the sample, i.e. under conditions similar to those in which dogs solve the same problem. The experimental cancer models were male BDF-f1 hybrid mice transplanted with hepatocarcinoma tissue, and similar mice transplanted with healthy liver tissue were used as controls. Our data show that both dogs and our proposed laser mass spectrometry method are able to detect both the entire spectrum of volatile organic compounds associated with the disease and minor changes in this spectrum during its course.
(© 2022. The Author(s).)
Databáze: MEDLINE
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