Dogs as carriers of virulent and resistant genotypes of Clostridioides difficile.

Autor: Finsterwalder SK; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria.; Clinical Unit of Internal Medicine Small Animals, University of Veterinary Medicine Vienna, Vienna, Austria., Loncaric I; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria., Cabal A; AGES - Austrian Agency for Health and Food Safety, Vienna, Austria., Szostak MP; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria., Barf LM; Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Jena, Germany.; Max Planck Institute for Science of Human History, Jena, Germany., Marz M; Faculty of Mathematics and Computer Science, Friedrich Schiller University Jena, Jena, Germany.; FLI Leibniz Institute for Age Research, Jena, Germany.; InfectoGnostics Research Campus Jena, Jena, Germany., Allerberger F; AGES - Austrian Agency for Health and Food Safety, Vienna, Austria., Burgener IA; Clinical Unit of Internal Medicine Small Animals, University of Veterinary Medicine Vienna, Vienna, Austria., Tichy A; Department of Biomedical Science, University of Veterinary Medicine Vienna, Vienna, Austria., Feßler AT; Department of Veterinary Medicine, Centre of Infection Medicine, Institute of Microbiology and Epizootics, Freie Universität Berlin, Berlin, Germany.; Department of Veterinary Medicine, Veterinary Centre for Resistance Research (TZR), Freie Universität Berlin, Berlin, Germany., Schwarz S; Department of Veterinary Medicine, Centre of Infection Medicine, Institute of Microbiology and Epizootics, Freie Universität Berlin, Berlin, Germany.; Department of Veterinary Medicine, Veterinary Centre for Resistance Research (TZR), Freie Universität Berlin, Berlin, Germany., Monecke S; InfectoGnostics Research Campus Jena, Jena, Germany.; Leibniz Institute of Photonic Technology (IPHT), Jena, Germany.; Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinik Dresden, Dresden, Germany., Ehricht R; InfectoGnostics Research Campus Jena, Jena, Germany.; Leibniz Institute of Photonic Technology (IPHT), Jena, Germany.; Institute of Physical Chemistry, Friedrich Schiller University Jena, Jena, Germany., Ruppitsch W; AGES - Austrian Agency for Health and Food Safety, Vienna, Austria., Spergser J; Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria., Künzel F; Clinical Unit of Internal Medicine Small Animals, University of Veterinary Medicine Vienna, Vienna, Austria.
Jazyk: angličtina
Zdroj: Zoonoses and public health [Zoonoses Public Health] 2022 Sep; Vol. 69 (6), pp. 673-681. Date of Electronic Publication: 2022 May 12.
DOI: 10.1111/zph.12956
Abstrakt: While previous research on zoonotic transmission of community-acquired Clostridioides difficile infection (CA-CDI) focused on food-producing animals, the present study aimed to investigate whether dogs are carriers of resistant and/or virulent C. difficile strains. Rectal swabs were collected from 323 dogs and 38 C. difficile isolates (11.8%) were obtained. Isolates were characterized by antimicrobial susceptibility testing, whole-genome sequencing (WGS) and a DNA hybridization assay. Multilocus sequence typing (MLST), core genome MLST (cgMLST) and screening for virulence and antimicrobial resistance genes were performed based on WGS. Minimum inhibitory concentrations for erythromycin, clindamycin, tetracycline, vancomycin and metronidazole were determined by E-test. Out of 38 C. difficile isolates, 28 (73.7%) carried genes for toxins. The majority of isolates belonged to MLST sequence types (STs) of clade I and one to clade V. Several isolates belonged to STs previously associated with human CA-CDI. However, cgMLST showed low genetic relatedness between the isolates of this study and C. difficile strains isolated from humans in Austria for which genome sequences were publicly available. Four isolates (10.5%) displayed resistance to three of the tested antimicrobial agents. Isolates exhibited resistance to erythromycin, clindamycin, tetracycline and metronidazole. These phenotypic resistances were supported by the presence of the resistance genes erm(B), cfr(C) and tet(M). All isolates were susceptible to vancomycin. Our results indicate that dogs may carry virulent and antimicrobial-resistant C. difficile strains.
(© 2022 The Authors. Zoonoses and Public Health published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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