Emergence of SARS-CoV-2 Spike Mutations during Prolonged Infection in Immunocompromised Hosts.

Autor:	Ko KKK; Department of Microbiology, Singapore General Hospitalgrid.163555.1, Singapore, Singapore.; Department of Molecular Pathology, Singapore General Hospitalgrid.163555.1, Singapore, Singapore.; Genome Institute of Singapore, Agency for Science, Technology, and Research, Singapore, Singapore.; Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.; Duke NUS Medical School, National University of Singapore, Singapore, Singapore., Yingtaweesittikul H; Advanced Research Center for Computational Simulation, Chiang Mai Universitygrid.7132.7, Chiang Mai, Thailand., Tan TT; Duke NUS Medical School, National University of Singapore, Singapore, Singapore.; Department of Infectious Diseases, Singapore General Hospitalgrid.163555.1, Singapore, Singapore., Wijaya L; Duke NUS Medical School, National University of Singapore, Singapore, Singapore.; Department of Infectious Diseases, Singapore General Hospitalgrid.163555.1, Singapore, Singapore., Cao DY; Department of Microbiology, Singapore General Hospitalgrid.163555.1, Singapore, Singapore., Goh SS; Department of Microbiology, Singapore General Hospitalgrid.163555.1, Singapore, Singapore., Abdul Rahman NB; Department of Microbiology, Singapore General Hospitalgrid.163555.1, Singapore, Singapore., Chan KXL; Department of Molecular Pathology, Singapore General Hospitalgrid.163555.1, Singapore, Singapore., Tay HM; Department of Haematology, Singapore General Hospitalgrid.163555.1, Singapore, Singapore., Sim JHC; Department of Microbiology, Singapore General Hospitalgrid.163555.1, Singapore, Singapore.; Duke NUS Medical School, National University of Singapore, Singapore, Singapore., Chan KS; Department of Molecular Pathology, Singapore General Hospitalgrid.163555.1, Singapore, Singapore.; Duke NUS Medical School, National University of Singapore, Singapore, Singapore., Oon LLE; Department of Molecular Pathology, Singapore General Hospitalgrid.163555.1, Singapore, Singapore.; Duke NUS Medical School, National University of Singapore, Singapore, Singapore., Nagarajan N; Genome Institute of Singapore, Agency for Science, Technology, and Research, Singapore, Singapore.; Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore., Suphavilai C; Genome Institute of Singapore, Agency for Science, Technology, and Research, Singapore, Singapore.
Jazyk:	angličtina
Zdroj:	Microbiology spectrum [Microbiol Spectr] 2022 Jun 29; Vol. 10 (3), pp. e0079122. Date of Electronic Publication: 2022 May 11.
DOI:	10.1128/spectrum.00791-22
Abstrakt:	Immunocompromised hosts with prolonged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections have been implicated in the emergence of highly mutated SARS-CoV-2 variants. Spike mutations are of particular concern because the spike protein is a key target for vaccines and therapeutics for SARS-CoV-2. Here, we report the emergence of spike mutations in two immunocompromised patients with persistent SARS-CoV-2 reverse transcription (RT)-PCR positivity (>90 days). Whole-genome sequence analysis of samples obtained before and after coronavirus disease 2019 (COVID-19) treatment demonstrated the development of partial therapeutic escape mutations and increased intrahost SARS-CoV-2 genome diversity over time. This case series thus adds to the accumulating evidence that immunocompromised hosts with persistent infections are important sources of SARS-CoV-2 genome diversity and, in particular, clinically important spike protein diversity. IMPORTANCE The emergence of clinically important mutations described in this report highlights the need for sustained vigilance and containment measures when managing immunocompromised patients with persistent COVID-19. Even as jurisdictions across the globe start lifting pandemic control measures, immunocompromised patients with persistent COVID-19 constitute a unique group that requires close genomic monitoring and enhanced infection control measures, to ensure early detection and containment of mutations and variants of therapeutic and public health importance.
Databáze:	MEDLINE
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