| Autor: |
de Jong TV; Department of Pharmacology, Addiction Science and Toxicology, University of Tennessee Health Science Center, Memphis, Tennessee., Chen H; Department of Pharmacology, Addiction Science and Toxicology, University of Tennessee Health Science Center, Memphis, Tennessee., Brashear WA; Texas A&M Institute for Genome Sciences and Society, Texas A&M University, College Station, Texas., Kochan KJ; Texas A&M Institute for Genome Sciences and Society, Texas A&M University, College Station, Texas., Hillhouse AE; Texas A&M Institute for Genome Sciences and Society, Texas A&M University, College Station, Texas., Zhu Y; Center for Human Genetics, Brown Foundation Institute of Molecular Medicine, University of Texas McGovern School of Medicine, Houston, Texas., Dhande IS; Center for Human Genetics, Brown Foundation Institute of Molecular Medicine, University of Texas McGovern School of Medicine, Houston, Texas., Hudson EA; Department of Biochemistry and Molecular Genetics, University of Louisville, Louisville, Kentucky., Sumlut MH; Department of Biochemistry and Molecular Genetics, University of Louisville, Louisville, Kentucky., Smith ML; Department of Biochemistry and Molecular Genetics, University of Louisville, Louisville, Kentucky., Kalbfleisch TS; Department of Veterinary Science, College of Agriculture, Food, and Environment, University of Kentucky, Lexington, Kentucky., Doris PA; Center for Human Genetics, Brown Foundation Institute of Molecular Medicine, University of Texas McGovern School of Medicine, Houston, Texas. |
| Abstrakt: |
Rat genomic tools have been slower to emerge than for those of humans and mice and have remained less thorough and comprehensive. The arrival of a new and improved rat reference genome, mRatBN7.2, in late 2020 is a welcome event. This assembly, like predecessor rat reference assemblies, is derived from an inbred Brown Norway rat. In this "user" survey we hope to provide other users of this assembly some insight into its characteristics and some assessment of its improvements as well as a few caveats that arise from the unique aspects of this assembly. mRatBN7.2 was generated by the Wellcome Sanger Institute as part of the large Vertebrate Genomes Project. This rat assembly has now joined human, mouse, chicken, and zebrafish in the National Center for Biotechnology Information (NCBI)'s Genome Reference Consortium, which provides ongoing curation of the assembly. Here we examine the technical procedures by which the assembly was created and assess how this assembly constitutes an improvement over its predecessor. We also indicate the technical limitations affecting the assembly, providing illustrations of how these limitations arise and the impact that results for this reference assembly. |
