Nanofibrous artificial skin substitute composed of mPEG-PCL grafted gelatin/hyaluronan/chondroitin sulfate/sericin for 2 nd degree burn care: in vitro and in vivo study.
| Autor: | Bhowmick S; Max-Bergmann Center of Biomaterials Dresden, Technische Universität Dresden Budapester Straße 27 01069 Dresden Germany.; Centre for Biomedical Engineering, Indian Institute of Technology Delhi Hauz Khas New Delhi 110016 India veenak@iitd.ac.in veenak_iitd@yahoo.com +91-11-26591041 +91-11-26596187.; Biomedical Engineering Unit, All India Institute of Medical Sciences New Delhi 110029 India., Thanusha AV; Centre for Biomedical Engineering, Indian Institute of Technology Delhi Hauz Khas New Delhi 110016 India veenak@iitd.ac.in veenak_iitd@yahoo.com +91-11-26591041 +91-11-26596187.; Biomedical Engineering Unit, All India Institute of Medical Sciences New Delhi 110029 India., Kumar A; Centre for Biomedical Engineering, Indian Institute of Technology Delhi Hauz Khas New Delhi 110016 India veenak@iitd.ac.in veenak_iitd@yahoo.com +91-11-26591041 +91-11-26596187.; Biomedical Engineering Unit, All India Institute of Medical Sciences New Delhi 110029 India., Scharnweber D; Max-Bergmann Center of Biomaterials Dresden, Technische Universität Dresden Budapester Straße 27 01069 Dresden Germany., Rother S; Max-Bergmann Center of Biomaterials Dresden, Technische Universität Dresden Budapester Straße 27 01069 Dresden Germany., Koul V; Centre for Biomedical Engineering, Indian Institute of Technology Delhi Hauz Khas New Delhi 110016 India veenak@iitd.ac.in veenak_iitd@yahoo.com +91-11-26591041 +91-11-26596187.; Biomedical Engineering Unit, All India Institute of Medical Sciences New Delhi 110029 India. |
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| Jazyk: | angličtina |
| Zdroj: | RSC advances [RSC Adv] 2018 May 03; Vol. 8 (30), pp. 16420-16432. Date of Electronic Publication: 2018 May 03 (Print Publication: 2018). |
| DOI: | 10.1039/c8ra01489b |
| Abstrakt: | The aim of this study was to investigate the efficacy of a skin substitute composed of mPEG-PCL-grafted-gelatin (Bio-Syn)/hyaluronan/chondroitin sulfate/sericin and to study its in vitro biocompatibility with human fibroblasts, human keratinocytes and hMSCs in terms of cellular adhesion and proliferation (∼5-6 fold). mPEG-PCL was grafted into a gelatin backbone via a Michael addition reaction to prepare Bio-Syn and it was characterized using ATR-FTIR, 1 H NMR and TNBS assay. Additionally, keratinocyte-hMSC contact co-culture studies showed that Bio-Syn composite scaffolds loaded with sericin promote hMSCs' epithelial differentiation with regard to qRT-PCR gene expression (ΔNp63α and keratin 14) and expression of various epithelial markers (Pan-cytokeratin, ΔNp63α and keratin 14). In vivo efficacy studies on a 2 nd degree burn wound model in Wistar rats showed an improved rate of wound contraction, histology (H&E and Van Gieson's staining) and pro-healing marker (hexosamine, hydroxyproline, etc. ) expression in granular tissue compared to using the commercial dressing Neuskin™ and a cotton gauze control. Competing Interests: The authors declare no competing financial interest. (This journal is © The Royal Society of Chemistry.) |
| Databáze: | MEDLINE |
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