Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle.
| Autor: | Zaki AJ; School of Physics and Astronomy, Cardiff University Cardiff CF24 3AA UK ElliottM@cardiff.ac.uk., Hartley AM; School of Biosciences, Cardiff University CF10 3AX UK JonesDD@cardiff.ac.uk., Reddington SC; School of Biosciences, Cardiff University CF10 3AX UK JonesDD@cardiff.ac.uk., Thomas SK; School of Physics and Astronomy, Cardiff University Cardiff CF24 3AA UK ElliottM@cardiff.ac.uk., Watson P; School of Biosciences, Cardiff University CF10 3AX UK JonesDD@cardiff.ac.uk., Hayes A; School of Biosciences, Cardiff University CF10 3AX UK JonesDD@cardiff.ac.uk., Moskalenko AV; School of Physics and Astronomy, Cardiff University Cardiff CF24 3AA UK ElliottM@cardiff.ac.uk., Craciun MF; Centre for Graphene Science, University of Exeter EX4 4QF UK., Macdonald JE; School of Physics and Astronomy, Cardiff University Cardiff CF24 3AA UK ElliottM@cardiff.ac.uk., Jones DD; School of Biosciences, Cardiff University CF10 3AX UK JonesDD@cardiff.ac.uk., Elliott M; School of Physics and Astronomy, Cardiff University Cardiff CF24 3AA UK ElliottM@cardiff.ac.uk. |
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| Jazyk: | angličtina |
| Zdroj: | RSC advances [RSC Adv] 2018 Feb 05; Vol. 8 (11), pp. 5768-5775. Date of Electronic Publication: 2018 Feb 05 (Print Publication: 2018). |
| DOI: | 10.1039/c7ra11166e |
| Abstrakt: | We have created modified protein variants by introducing a non-canonical amino acid p -azido-l-phenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b Competing Interests: There are no conflicts of interest to declare. (This journal is © The Royal Society of Chemistry.) |
| Databáze: | MEDLINE |
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