Metabarcoding dataset on the elicitation of Soybean and Mungbean using Ragi Tape as elicitors for enhancing secondary metabolites production.

Autor:	Hermanto FE; Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University. Jl. Veteran Malang 65145, East Java, Indonesia., Warsito W; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Brawijaya University. Jl. Veteran Malang 65145, East Java, Indonesia.; Essential Oil's Institute, Brawijaya University, Jl Veteran Malang 65145, East Java, Indonesia., Rifa'i M; Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University. Jl. Veteran Malang 65145, East Java, Indonesia.; Biosystem Study Center, Brawijaya University, Jl. Veteran Malang 65145, East Java, Indonesia., Widodo N; Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University. Jl. Veteran Malang 65145, East Java, Indonesia.; Biosystem Study Center, Brawijaya University, Jl. Veteran Malang 65145, East Java, Indonesia., Jatmiko YD; Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University. Jl. Veteran Malang 65145, East Java, Indonesia.
Jazyk:	angličtina
Zdroj:	Data in brief [Data Brief] 2022 Apr 26; Vol. 42, pp. 108209. Date of Electronic Publication: 2022 Apr 26 (Print Publication: 2022).
DOI:	10.1016/j.dib.2022.108209
Abstrakt:	Ragi Tape (RT) is commonly used as the microbial starter for various Indonesian traditional food. Consist of diverse microbial populations, RT has excellent agents as elicitors to augment the bioactive compounds in Soybean (SB) and Mungbean (MB). Metabarcoding analysis has shown to identify the microbial population involved in elicitation using RT. Inoculated RT from SB and MB were collected to extract the microbial DNA for the post-elicitation group. In comparison, DNA extraction from powdered RT was conducted as a pre-elicitation group. The total DNA were then sequenced by using the MiSeq Illumina platform with 16S rDNA gene region V3-V4 and 18S rDNA gene region V4 as a biomarker for bacterial and fungal identification, correspondingly. The obtained raw-data sequences were then analyzed using QIIME2 pipeline. According to the number of the acquired sequences, the 18S sequencing yielded more DNA strands than the 16S sequencing. However, the number of assigned OTUs was higher in 16S sequences than 18S sequences. From the perspective of the sample, RT has larger distinctive taxa, which were not identified in other samples. This metagenome data will provide fundamental information on RT employment in the elicitation process and further understanding of elicitation mechanisms using RT as biotic elicitors. The data is available at the BioProject database under the NCBI domain with accession no. PRJNA767401. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper. (© 2022 The Author(s). Published by Elsevier Inc.)
Databáze:	MEDLINE
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