Metagenomic sequencing characterizes a wide diversity of viruses in field mosquito samples in Nigeria.

Autor: Oguzie JU; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun, Nigeria.; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede, Osun, Nigeria., Nwangwu UC; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Oluniyi PE; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun, Nigeria.; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede, Osun, Nigeria., Olumade TJ; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun, Nigeria.; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede, Osun, Nigeria., George UE; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun, Nigeria.; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede, Osun, Nigeria., Kazeem A; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun, Nigeria.; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede, Osun, Nigeria., Bankole BE; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun, Nigeria.; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede, Osun, Nigeria., Brimmo FO; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun, Nigeria., Asadu CC; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Chukwuekezie OC; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Ochu JC; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Makwe CO; Médecins Sans Frontières (MSF Belgium), Bruxelles, Belgium., Dogunro FA; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Onwude CO; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Nwachukwu WE; Nigeria Center for Disease Control, Abuja, Nigeria., Ezihe EK; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Okonkwo GK; Médecins Sans Frontières (MSF Belgium), Bruxelles, Belgium., Umazi NE; Médecins Sans Frontières (MSF Belgium), Bruxelles, Belgium., Maikere J; Médecins Sans Frontières (MSF Belgium), Bruxelles, Belgium., Agashi NO; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Eloy EI; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Anokwu SO; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Okoronkwo AI; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Nwosu EM; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Etiki SO; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Ngwu IM; National Arbovirus and Vectors Research Centre (NAVRC), Enugu, Enugu, Nigeria., Ihekweazu C; Nigeria Center for Disease Control, Abuja, Nigeria., Folarin OA; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun, Nigeria.; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede, Osun, Nigeria., Komolafe IOO; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun, Nigeria.; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede, Osun, Nigeria., Happi CT; African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun, Nigeria. happic@run.edu.ng.; Department of Biological Sciences, Faculty of Natural Sciences, Redeemer's University, Ede, Osun, Nigeria. happic@run.edu.ng.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 May 10; Vol. 12 (1), pp. 7616. Date of Electronic Publication: 2022 May 10.
DOI: 10.1038/s41598-022-11797-2
Abstrakt: Mosquito vectors are a tremendous public health threat. One in six diseases worldwide is vector-borne transmitted mainly by mosquitoes. In the last couple of years, there have been active Yellow fever virus (YFV) outbreaks in many settings in Nigeria, and nationwide, entomological surveillance has been a significant effort geared towards understanding these outbreaks. In this study, we used a metagenomic sequencing approach to characterize viruses present in vector samples collected during various outbreaks of Yellow fever (YF) in Nigeria between 2017 and 2020. Mosquito samples were grouped into pools of 1 to 50 mosquitoes, each based on species, sex and location. Twenty-five pools of Aedes spp and one pool of Anopheles spp collected from nine states were sequenced and metagenomic analysis was carried out. We identified a wide diversity of viruses belonging to various families in this sample set. Seven different viruses detected included: Fako virus, Phasi Charoen-like virus, Verdadero virus, Chaq like-virus, Aedes aegypti totivirus, cell fusing agent virus and Tesano Aedes virus. Although there are no reports of these viruses being pathogenic, they are an understudied group in the same families and closely related to known pathogenic arboviruses. Our study highlights the power of next generation sequencing in identifying Insect specific viruses (ISVs), and provide insight into mosquito vectors virome in Nigeria.
(© 2022. The Author(s).)
Databáze: MEDLINE
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