Gram-positive Staphylococcus aureus LTA promotes distinct memory-like effects in murine bone marrow neutrophils.

Autor: Lajqi T; Department of Neonatology, Heidelberg University Children's Hospital, 69120 Heidelberg, Germany., Frommhold D; Klinik für Kinderheilkunde und Jugendmedizin, 87700 Memmingen, Germany., Braun M; Department of Neonatology, Heidelberg University Children's Hospital, 69120 Heidelberg, Germany., Alexander Kranig S; Department of Neonatology, Heidelberg University Children's Hospital, 69120 Heidelberg, Germany., Pöschl J; Department of Neonatology, Heidelberg University Children's Hospital, 69120 Heidelberg, Germany., Hudalla H; Department of Neonatology, Heidelberg University Children's Hospital, 69120 Heidelberg, Germany. Electronic address: Hannes.Hudalla@med.uni-heidelberg.de.
Jazyk: angličtina
Zdroj: Cellular immunology [Cell Immunol] 2022 Jun; Vol. 376, pp. 104535. Date of Electronic Publication: 2022 May 04.
DOI: 10.1016/j.cellimm.2022.104535
Abstrakt: Neutrophils primarily act as first responders in acute infection and directly maintain inflammatory responses. However, a growing body of evidence suggests that neutrophils also bear the potential to mediate chronic inflammation by exhibiting memory-like features. We now asked whether bone marrow-derived murine neutrophils can be primed by lipoteichoic acid (LTA) from gram-positive S. aureus. We found that low-dose (1 ng/mL) LTA-priming promoted increased production of pro-inflammatory mediators (TNF-α, IL-6, ROS), whereas high-dose (10 µg/mL) priming resulted in opposing reactions marked by increased IL-10 and suppressed pro-inflammatory mediators upon a second stimulus. A similar pattern of pro-inflammatory activation (trained sensitivity) and anti-inflammatory properties (tolerance) was recapitulated in cellular functional in vitro assays (transmigration and phagocytosis). Priming by LTA correlated with TLR2/MyD88-mediated regulation of NFκB-p65 through intermediate PI3Ks/MAPK. Collectively, our data suggest a previously unknown capacity of neutrophils to be differentially primed by varying doses of LTA, endorsing memory-like features in neutrophils.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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