Inositol hexakisphosphate primes syndapin I/PACSIN 1 activation in endocytosis.

Autor: Shi Y; The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, 171 76, Stockholm, Sweden. yue.shi@ki.se., Zhao K; The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, 171 76, Stockholm, Sweden., Yang G; The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, 171 76, Stockholm, Sweden.; Jilin Academy of Traditional Chinese Medicine, Changchun, 130021, China., Yu J; The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, 171 76, Stockholm, Sweden., Li Y; National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, 130024, China., Kessels MM; Institute for Biochemistry I, Jena University Hospital/Friedrich Schiller University Jena, 07743, Jena, Germany., Yu L; The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, 171 76, Stockholm, Sweden., Qualmann B; Institute for Biochemistry I, Jena University Hospital/Friedrich Schiller University Jena, 07743, Jena, Germany., Berggren PO; The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, 171 76, Stockholm, Sweden. per-olof.berggren@ki.se.; Center for Diabetes and Metabolism Research, Division of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, 610041, China. per-olof.berggren@ki.se., Yang SN; The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital L1, 171 76, Stockholm, Sweden. shao-nian.yang@ki.se.; National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, 130024, China. shao-nian.yang@ki.se.
Jazyk: angličtina
Zdroj: Cellular and molecular life sciences : CMLS [Cell Mol Life Sci] 2022 May 09; Vol. 79 (6), pp. 286. Date of Electronic Publication: 2022 May 09.
DOI: 10.1007/s00018-022-04305-2
Abstrakt: Endocytosis is controlled by a well-orchestrated molecular machinery, where the individual players as well as their precise interactions are not fully understood. We now show that syndapin I/PACSIN 1 is expressed in pancreatic β cells and that its knockdown abrogates β cell endocytosis leading to disturbed plasma membrane protein homeostasis, as exemplified by an elevated density of L-type Ca 2+ channels. Intriguingly, inositol hexakisphosphate (InsP 6 ) activates casein kinase 2 (CK2) that phosphorylates syndapin I/PACSIN 1, thereby promoting interactions between syndapin I/PACSIN 1 and neural Wiskott-Aldrich syndrome protein (N-WASP) and driving β cell endocytosis. Dominant-negative interference with endogenous syndapin I/PACSIN 1 protein complexes, by overexpression of the syndapin I/PACSIN 1 SH3 domain, decreases InsP 6 -stimulated endocytosis. InsP 6 thus promotes syndapin I/PACSIN 1 priming by CK2-dependent phosphorylation, which endows the syndapin I/PACSIN 1 SH3 domain with the capability to interact with the endocytic machinery and thereby initiate endocytosis, as exemplified in β cells.
(© 2022. The Author(s).)
Databáze: MEDLINE
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