Well water sources simultaneous contamination with Cryptosporidium and Acanthamoeba in East-Southeast Asia and Acanthamoeba spp. in biofilms in the Philippines.

Autor: Masangkay FR; Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines. Electronic address: frmasangkay@ust.edu.ph., Milanez GD; Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila 1008, Philippines., Dionisio JD; Department of Medical Technology, Far Eastern University-Manila, Manila 1015, Philippines., Ormita LAG; Department of Psychology, Far Eastern University-Manila, Manila 1015, Philippines; Community Extension Services, Far Eastern University-Manila, Manila 1015, Philippines., Alvarez AV; Community Extension Services, Far Eastern University-Manila, Manila 1015, Philippines., Karanis P; University of Cologne, Faculty of Medical and University Hospital Cologne, Cologne 50923, Germany; Medical School, Department of Basic and Clinical Science, University of Nicosia, Nicosia, 2417, Cyprus. Electronic address: karanis.p@unic.ac.cy.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2022 Sep 01; Vol. 837, pp. 155752. Date of Electronic Publication: 2022 May 06.
DOI: 10.1016/j.scitotenv.2022.155752
Abstrakt: Cryptosporidium is the leading agent of waterborne parasitic protozoan outbreaks and is the second leading cause of infant mortality due to diarrhoea worldwide. Acanthamoeba spp. causes Acanthamoeba keratitis (AK) and a life-threatening condition known as granulomatous amoebic encephalitis (GAE). The present study aimed to assess the water quality of an indigenous and a rural community for waterborne parasitic protozoan contamination. Aquatic samples (n = 22) were processed by filtration of 500 mL portion through a 1.2 μm pore size glass microfiber filter and eluted for light microscopy, culture in non-nutrient agar, and PCR analysis. Overall, 36% (8/22) of the investigated aquatic samples were positive for either Cryptosporidium spp. oocysts (13%; 3/22) or Acanthamoeba spp., (36%; 8/22) or both (13%; 3/22). Cryptosporidium spp. oocysts were detected in 27% (3/11) of wet season samples only while Acanthamoeba spp. were detected in 18% (2/11) and 55% (6/11) of wet and dry season samples, respectively. Subsequently, molecular detection for Acanthamoeba species identified A. lenticulata and A. hatchetti with 98-99% BLAST similarity. This is the first report on the simultaneous contamination of Cryptosporidium and Acanthamoeba in well water sources in East-Southeast Asia, the first detection of Acanthamoeba spp. in biofilms in the Philippines, and the longest viability demonstrated for A. lenticulata in two-year-old water samples stored at room temperature.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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