Effects of Testosterone on Mixed-Muscle Protein Synthesis and Proteome Dynamics During Energy Deficit.

Autor:	Howard EE; Military Nutrit ion Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA 01760, USA.; Oak Ridge Institute for Science and Education, Oak Ridge, TN 37830, USA., Shankaran M; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA., Evans WJ; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA., Berryman CE; Military Nutrit ion Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA 01760, USA.; Oak Ridge Institute for Science and Education, Oak Ridge, TN 37830, USA.; Department of Nutrition, Food, and Exercise Sciences, Florida State University, Tallahassee, FL 32306, USA., Margolis LM; Military Nutrit ion Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA 01760, USA., Lieberman HR; Military Nutrit ion Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA 01760, USA., Karl JP; Military Nutrit ion Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA 01760, USA., Young AJ; Military Nutrit ion Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA 01760, USA., Montano MA; MyoSyntax Corporation, Worcester, MA 01605, USA.; Harvard Medical School, Boston, MA 02115, USA.; Brigham and Women's Hospital, Boston, MA 02115, USA., Matthews MD; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA., Bizieff A; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA., Nyangao E; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA., Mohammed H; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA., Harris MN; Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA., Hellerstein MK; Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720, USA., Rood JC; Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA., Pasiakos SM; Military Performance Division, U.S. Army Research Institute of Environmental Medicine, Natick, MA 01760, USA.
Jazyk:	angličtina
Zdroj:	The Journal of clinical endocrinology and metabolism [J Clin Endocrinol Metab] 2022 Jul 14; Vol. 107 (8), pp. e3254-e3263.
DOI:	10.1210/clinem/dgac295
Abstrakt:	Context: Effects of testosterone on integrated muscle protein metabolism and muscle mass during energy deficit are undetermined. Objective: The objective was to determine the effects of testosterone on mixed-muscle protein synthesis (MPS), proteome-wide fractional synthesis rates (FSR), and skeletal muscle mass during energy deficit. Design: This was a randomized, double-blind, placebo-controlled trial. Setting: The study was conducted at Pennington Biomedical Research Center. Participants: Fifty healthy men. Intervention: The study consisted of 14 days of weight maintenance, followed by a 28-day 55% energy deficit with 200 mg testosterone enanthate (TEST, n = 24) or placebo (PLA, n = 26) weekly, and up to 42 days of ad libitum recovery feeding. Main Outcome Measures: Mixed-MPS and proteome-wide FSR before (Pre), during (Mid), and after (Post) the energy deficit were determined using heavy water (days 1-42) and muscle biopsies. Muscle mass was determined using the D3-creatine dilution method. Results: Mixed-MPS was lower than Pre at Mid and Post (P < 0.0005), with no difference between TEST and PLA. The proportion of individual proteins with numerically higher FSR in TEST than PLA was significant by 2-tailed binomial test at Post (52/67; P < 0.05), but not Mid (32/67; P > 0.05). Muscle mass was unchanged during energy deficit but was greater in TEST than PLA during recovery (P < 0.05). Conclusions: The high proportion of individual proteins with greater FSR in TEST than PLA at Post suggests exogenous testosterone exerted a delayed but broad stimulatory effect on synthesis rates across the muscle proteome during energy deficit, resulting in muscle mass accretion during subsequent recovery. (Published by Oxford University Press on behalf of the Endocrine Society 2022.)
Databáze:	MEDLINE
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