Kif9 is an active kinesin motor required for ciliary beating and proximodistal patterning of motile axonemes.

Autor:	Konjikusic MJ; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA.; Department of Pediatrics, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd, The University of Texas at Austin, Dell Medical School, Austin, TX 78712, USA.; Department of Nutritional Sciences, 200 W 24th Street, The University of Texas at Austin, Austin, TX 78712, USA., Lee C; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA., Yue Y; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Shrestha BD; Department of Physics, University of Miami, Coral Gables, FL 33146, USA., Nguimtsop AM; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA., Horani A; Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63130, USA.; Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA., Brody S; Department of Medicine, Washington University School of Medicine, St. Louis, MO, 63110, USA., Prakash VN; Department of Physics, University of Miami, Coral Gables, FL 33146, USA.; Department of Biology and Department of Marine Biology and Ecology, University of Miami, Coral Gables, FL 33146,USA., Gray RS; Department of Pediatrics, Dell Pediatric Research Institute, 1400 Barbara Jordan Blvd, The University of Texas at Austin, Dell Medical School, Austin, TX 78712, USA.; Department of Nutritional Sciences, 200 W 24th Street, The University of Texas at Austin, Austin, TX 78712, USA., Verhey KJ; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA., Wallingford JB; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA.
Jazyk:	angličtina
Zdroj:	Journal of cell science [J Cell Sci] 2023 Mar 01; Vol. 136 (5). Date of Electronic Publication: 2022 Jun 22.
DOI:	10.1242/jcs.259535
Abstrakt:	Most motile cilia have a stereotyped structure of nine microtubule outer doublets and a single central pair of microtubules. The central pair of microtubules are surrounded by a set of proteins, termed the central pair apparatus. A specific kinesin, Klp1 projects from the central pair and contributes to ciliary motility in Chlamydomonas. The vertebrate ortholog, Kif9, is required for beating in mouse sperm flagella, but the mechanism of Kif9/Klp1 function remains poorly defined. Here, using Xenopus epidermal multiciliated cells, we show that Kif9 is necessary for ciliary motility and the proper distal localization of not only central pair proteins, but also radial spokes and dynein arms. In addition, single-molecule assays in vitro reveal that Xenopus Kif9 is a long-range processive motor, although it does not mediate long-range movement in ciliary axonemes in vivo. Together, our data suggest that Kif9 is integral for ciliary beating and is necessary for proper axonemal distal end integrity. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2022. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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