Biochemical and molecular diagnosis of different tomato cultivars susceptible and resistant to Tuta absoluta (Meyrick) infestation.

Autor:	El-Sitiny MFA; Plant Protection Department, Agricultural Faculty, Zagazig University, Egypt., M Omar H; Plant Protection Department, Agricultural Faculty, Zagazig University, Egypt., El-Shehawi AM; Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia., Elseehy MM; Department of Genetics, Faculty of Agriculture, University of Alexandria, Alexandria 21545, Egypt., El-Tahan AM; Plant Production Department, Arid Lands Cultivation Research Institute, The City of Scientific Research and Technological Applications, SRTA-City. Borg El Arab, Alexandria, Egypt., El-Saadony MT; Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt., Selem GS; Plant Protection Department, Agricultural Faculty, Zagazig University, Egypt.
Jazyk:	angličtina
Zdroj:	Saudi journal of biological sciences [Saudi J Biol Sci] 2022 Apr; Vol. 29 (4), pp. 2904-2910. Date of Electronic Publication: 2022 Jan 15.
DOI:	10.1016/j.sjbs.2022.01.024
Abstrakt:	Resistant plant cultivars which used in breeding programs are considered one of the modern integrated management programs to reduce the usage of synthetic insecticides and environmental contamination the present study aimed to characterize the resistant and susceptible tomato cultivars to Tuta absoluta based on biochemical and molecular levels, in Egypt. The biochemical characters of the tested tomato cultivars (tomato- 86, tomato- Alissa, tomato- Fayarouz, tomato- Omniya, tomato- 036, tomato- GS) were determined colorimetrically and characterized by using native- polyacrylamide gel electrophoresis (PAGE) and agarose gel. Our results showed that there were variations highly significant in all biochemical constituents of the resistant tomato cultivar (tomato- 86) compared with the susceptible one (tomato- GS). Also, native-(PAGE) for peroxidase (POD) isoenzymes techniques of the tested tomato cultivars showed variations in protein band numbers and densities in tomato-86 resistant compared with tomato-GS susceptible to Tuta absoluta infestation. The correlation coefficient between total phenols and peroxidases in infested tomato leaves and percentages of damaged leaves with the tested insect pest was negative and highly significant, while in case of total proteins and reducing sugars in infested tomato leaves as well as lycopene contents in infested tomato fruits was positive, highly significant and significant, respectively. The correlation coefficient between tomato yield means and the infested fruit percentage with T. absoluta larvae was negative and highly significant. Respecting molecular diagnosis random amplified polymorphism DNA- polymerase chain reaction (RAPD- PCR), the results demonstrated that the presence of polymorphism in the resistant tomato cultivar (tomato- 86) compared with (tomato- GS), the most susceptible to the tested insect pest infestation. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2022 The Authors.)
Databáze:	MEDLINE
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