Novel cationic tannin/glycosaminoglycan-based polyelectrolyte multilayers promote stem cells adhesion and proliferation.

Autor: da Câmara PCF; Laboratory of Petroleum Research, LAPET, Institute of Chemistry, Federal University of Rio Grande do Norte, UFRN 59078-970 Natal RN Brazil.; Department of Chemical and Biological Engineering, Colorado State University 1370 Campus Delivery Fort Collins 80523 CO USA., Balaban RC; Laboratory of Petroleum Research, LAPET, Institute of Chemistry, Federal University of Rio Grande do Norte, UFRN 59078-970 Natal RN Brazil., Hedayati M; Department of Chemical and Biological Engineering, Colorado State University 1370 Campus Delivery Fort Collins 80523 CO USA., Popat KC; Department of Mechanical Engineering, Colorado State University 1374 Campus Delivery Fort Collins CO 80523 USA., Martins AF; Laboratory of Materials, Macromolecules and Composites, Federal University of Technology Apucarana PR 86812-460 Brazil affmmartins@gmail.com.; Department of Chemical and Biological Engineering, Colorado State University 1370 Campus Delivery Fort Collins 80523 CO USA., Kipper MJ; Department of Chemical and Biological Engineering, Colorado State University 1370 Campus Delivery Fort Collins 80523 CO USA.
Jazyk: angličtina
Zdroj: RSC advances [RSC Adv] 2019 Aug 19; Vol. 9 (44), pp. 25836-25846. Date of Electronic Publication: 2019 Aug 19 (Print Publication: 2019).
DOI: 10.1039/c9ra03903a
Abstrakt: Modifying materials with biocompatible surface coatings is an important method for controlling cell responses to biomaterials. In this work, tanfloc (TN), a cationic tannin-derivative polymer was assembled with heparin (HEP) and chondroitin sulfate (CS), using the layer-by-layer (LbL) approach, to build polyelectrolyte multilayers (PEMs) and to design cytocompatible coatings. LbL deposition was monitored through Fourier-transform surface plasmon resonance, and characterized by X-ray photoelectron spectroscopy, atomic force microscopy, and contact angle measurements. The response of human adipose-derived stem cells (ADSCs) was evaluated in vitro . All of the TN-containing PEMs exhibit cytocompatibility and support adhesion, proliferation, and the spreading of ADSCs after 7 days of culture. HEP-TN PEM assembly with 11 layers (HEP-TN 11 ) supports the greatest rate of cell proliferation. When TN is the terminal layer of the PEM, the surfaces promote the spreading of ADSCs, indicating that the surface charge and PEM terminal layer are key determinants of the microenvironmental niche that control cellular response. The promotion of stem cell attachment and proliferation makes these surface coatings potentially useful for biomedical implants and regenerative medicine.
Competing Interests: There are no conflicts to declare.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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