Molecular detection and analysis of virulence genes in multi-drug resistant Escherichia coli from infected broilers.

Autor:	Azim KF; Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh., Somana SR; Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh., Hasan MK; Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh., Foysal MJ; Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh.; School of Molecular and Life Sciences, Curtin University, Perth, Australia., Ali MH; Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh., Chowdhury TA; Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh., Hossain MN; Department of Microbial Biotechnology, Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet, Bangladesh.
Jazyk:	angličtina
Zdroj:	Veterinary research forum : an international quarterly journal [Vet Res Forum] 2021 Dec; Vol. 12 (4), pp. 505-510. Date of Electronic Publication: 2021 Dec 15.
DOI:	10.30466/vrf.2020.115921.2762
Abstrakt:	Escherichia coli associated infections are major threats in poultry industry owing to severe economic losses each year. This study was conducted to identify E. coli isolates, to evaluate their antibiotic sensitivity and to find out their virulence patterns from infected broilers of Sylhet city in Bangladesh. Using polymerase chain reaction, a total 20 isolates were identified as E. coli from 11 chickens, exhibiting symptoms like colibacillosis and/or diarrhea. All isolates were positive for type-1 fimbrial adhesion ( fimH ), followed by putative avian hemolysin ( hlyF ) in 17 isolates; while none of the isolates was amplified with intimin ( eaeA ). Among 10 tested antibiotics, 100% of the isolates (n = 20) showed resistance to ampicillin, amoxicillin and tetra-cycline; but they were 100% sensitive to gentamicin. Organ specific correlations of antibiotic sensitivity were obtained among the isolates through principal component analysis (PCA) and Agglomerative Hierarchical Clustering (AHC). The 16S rRNA data of two multi-drug resistant isolates revealed closed clustering with clinical E. coli strains which could be indication of their zoonotic potential. In conclusion, the results depict higher prevalence of fimH and hlyF genes and drug resistance patterns of E. coli isolates from broilers in Sylhet city of Bangladesh. Competing Interests: The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by the authors. (© 2021 Urmia University. All rights reserved.)
Databáze:	MEDLINE
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