An ionophore-based persistent luminescent 'Glow Sensor' for sodium detection.
| Autor: | Ferris MS; Chemical and Biological Engineering Department, Colorado School of Mines Golden CO 80401 USA kcash@mines.edu., Behr MR; Chemical and Biological Engineering Department, Colorado School of Mines Golden CO 80401 USA kcash@mines.edu., Cash KJ; Chemical and Biological Engineering Department, Colorado School of Mines Golden CO 80401 USA kcash@mines.edu.; Quantitative Biosciences and Engineering, Colorado School of Mines Golden CO 80401 USA. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | RSC advances [RSC Adv] 2019 Oct 15; Vol. 9 (56), pp. 32821-32825. Date of Electronic Publication: 2019 Oct 15 (Print Publication: 2019). |
| DOI: | 10.1039/c9ra05313a |
| Abstrakt: | Optical sensors have numerous positive attributes such as low invasiveness, miniaturizability, biocompatibility, and ease of signal transduction. Recently, there has been a strong research focus on using phosphorescent readout mechanisms, specifically from long-lifetime phosphorescent or 'persistent luminescence' particles, for in vitro and in vivo sensors. Persistent luminescence readouts can avoid cellular autofluorescence during biological monitoring, leading to an improved signal-to-noise ratio over a more traditional fluorescence readout. In this study, we show for the first time an ionophore-based optical bulk optode sensor that utilizes persistent luminescence microparticles for ion detection. To achieve this, we combined long-lifetime strontium aluminate-based 'glow-in-the-dark' microparticles with a non-fluorescent pH-responsive dye in a hydrophobic plasticized polymer membrane along with traditional ionophore-based optical sensor components to create a phosphorescent 'Glow Sensor'. The non-fluorescent pH indicator dye gates the strontium aluminate luminescence signal so that it decreases in magnitude with increasing sodium concentration. We characterized the Glow Sensor in terms of emission lifetime, dynamic range, response time, reversibility, selectivity, and stability. Competing Interests: There are no conflicts of interest to declare. (This journal is © The Royal Society of Chemistry.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|