Clinical training and validation of the LeukoScope: a low-cost, point-of-care device to perform white blood cell and neutrophil counts.

Autor:	Majors CE; Department of Bioengineering, Rice University 6100 Main Street, MS-142 Houston TX 77005 USA rkortum@rice.edu +1 713 348 3823., Pawlowski ME; Department of Bioengineering, Rice University 6100 Main Street, MS-142 Houston TX 77005 USA rkortum@rice.edu +1 713 348 3823., Burke DC; Department of Bioengineering, Rice University 6100 Main Street, MS-142 Houston TX 77005 USA rkortum@rice.edu +1 713 348 3823., Tkaczyk TS; Department of Bioengineering, Rice University 6100 Main Street, MS-142 Houston TX 77005 USA rkortum@rice.edu +1 713 348 3823., Rieber A; Department of General Oncology, The University of Texas MD Anderson Cancer Center Houston TX 77030 USA., Richards-Kortum R; Department of Bioengineering, Rice University 6100 Main Street, MS-142 Houston TX 77005 USA rkortum@rice.edu +1 713 348 3823.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2019 Aug 30; Vol. 9 (47), pp. 27324-27333. Date of Electronic Publication: 2019 Aug 30 (Print Publication: 2019).
DOI:	10.1039/c9ra06433h
Abstrakt:	A white blood cell (WBC) count with partial differential is an important clinical laboratory test. However, current methods to perform a WBC count and differential are difficult to use at the point of care or too expensive for use in low-resource settings. To meet this need, we developed the LeukoScope: a low-cost system to measure a WBC and neutrophil count from a single drop of blood at the point of care. The LeukoScope is battery powered and has a sample-to-answer time of <5 minutes. A drop of blood from a finger stick is added to a LeukoScope sample cartridge where pre-dried acridine orange fluorescently stains WBCs. The cartridge is then inserted into the LeukoScope reader where a portable fluorescence microscope captures a color image of the sample, which is analyzed to report results to the user. The LeukoScope system was tested at the point of care using fingerprick samples collected from 105 general oncology patients in Houston, TX. Performance of the LeukoScope was compared to that of a HemoCue WBC DIFF performed using the same fingerprick sample; clinical laboratory analysis of a venous blood draw was used as the gold standard in all cases. Bland-Altman analysis showed that the LeukoScope and HemoCue WBC DIFF had similar accuracy for measurement of WBC and neutrophil counts as compared to the gold standard. Seven out of eight patients with abnormal WBC count values were correctly identified using the LeukoScope, while six out of eight were correctly identified using the HemoCue WBC DIFF. Five out of six patients with abnormal neutrophil counts were correctly identified using the LeukoScope, while six of six were correctly identified using the HemoCue WBC DIFF. Competing Interests: The authors have no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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