Snf1p/Hxk2p/Mig1p pathway regulates hexose transporters transcript levels, affecting the exponential growth and mitochondrial respiration of Saccharomyces cerevisiae.

Autor: Carrillo-Garmendia A; Universidad Politécnica de Guanajuato, Av. Universidad Sur #1001, Cortazar, Guanajuato 38496, Mexico., Martinez-Ortiz C; Universidad Politécnica de Guanajuato, Av. Universidad Sur #1001, Cortazar, Guanajuato 38496, Mexico., Martinez-Garfias JG; Tecnológico Nacional de México/ Instituto Tecnológico Superior de Ciudad Hidalgo, Av. Ing. Carlos Rojas Gutiérrez #2120, Ciudad Hidalgo, Michoacán 61100, Mexico., Suarez-Sandoval SE; Tecnológico Nacional de México/ Instituto Tecnológico Superior de Ciudad Hidalgo, Av. Ing. Carlos Rojas Gutiérrez #2120, Ciudad Hidalgo, Michoacán 61100, Mexico., González-Hernández JC; Tecnológico Nacional de México/ Instituto Tecnológico de Morelia, Av. Tecnológico de Morelia, Morelia, Michoacán 58030, Mexico., Nava GM; Universidad Autónoma de Querétaro, Cerro de las Campanas, Santiago de Querétaro, Qro 76010, Mexico., Dufoo-Hurtado MD; Universidad Politécnica de Guanajuato, Av. Universidad Sur #1001, Cortazar, Guanajuato 38496, Mexico., Madrigal-Perez LA; Tecnológico Nacional de México/ Instituto Tecnológico Superior de Ciudad Hidalgo, Av. Ing. Carlos Rojas Gutiérrez #2120, Ciudad Hidalgo, Michoacán 61100, Mexico. Electronic address: lmadrigal@cdhidalgo.tecnm.mx.
Jazyk: angličtina
Zdroj: Fungal genetics and biology : FG & B [Fungal Genet Biol] 2022 Jul; Vol. 161, pp. 103701. Date of Electronic Publication: 2022 May 05.
DOI: 10.1016/j.fgb.2022.103701
Abstrakt: The Crabtree effect molecular regulation comprehension could help to improve ethanol production with biotechnological purposes and a better understanding of cancer etiology due to its similarity with the Warburg effect. Snf1p/Hxk2p/Mig1p pathway has been linked with the transcriptional regulation of the hexose transporters and phenotypes associated with the Crabtree effect. Nevertheless, direct evidence linking the genetic control of the hexose transporters with modulation of the Crabtree effect phenotypes by the Snf1p/Hxk2p/Mig1p pathway is still lacking. In this sense, we provide evidence that SNF1 and HXK2 genes deletion affects exponential growth, mitochondrial respiration, and transcript levels of hexose transporters in a glucose-dependent manner. The Vmax of the hexose transporters with the high transcript levels was correlated positively with the exponential growth and negatively with the mitochondrial respiration. HXT2 gene transcript levels were the most affected by the deletion of the SNF1/HXK2/MIG1 pathway. Deleting the orthologous genes SNF1 and HXK2 in Kluyveromyces marxianus (Crabtree negative yeast) has an opposite effect compared to Saccharomyces cerevisiae in growth and mitochondrial respiration. Overall, these results indicate that the SNF1/HXK2/MIG1 pathway regulates transcript levels of the hexose transporters, which shows an association with the exponential growth and mitochondrial respiration in a glucose-dependent manner.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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