Monogalactosyl diacylglycerol synthase 3 affects phosphate utilization and acquisition in rice.

Autor:	Verma L; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India., Bhadouria J; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India., Bhunia RK; National Agri-Food Biotechnology Institute (NABI), Mohali, Punjab, India.; Plant Science Department, Rothamsted Research, Harpenden, Hertfordshire, UK., Singh S; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India., Panchal P; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India., Bhatia C; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India., Eastmond PJ; Plant Science Department, Rothamsted Research, Harpenden, Hertfordshire, UK., Giri J; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India.
Jazyk:	angličtina
Zdroj:	Journal of experimental botany [J Exp Bot] 2022 Aug 11; Vol. 73 (14), pp. 5033-5051.
DOI:	10.1093/jxb/erac192
Abstrakt:	Galactolipids are essential to compensate for the loss of phospholipids by 'membrane lipid remodelling' in plants under phosphorus (P) deficiency conditions. Monogalactosyl diacylglycerol (MGDG) synthases catalyse the synthesis of MGDG which is further converted into digalactosyl diacylglycerol (DGDG), later replacing phospholipids in the extraplastidial membranes. However, the roles of these enzymes are not well explored in rice. In this study, the rice MGDG synthase 3 gene (OsMGD3) was identified and functionally characterized. We showed that the plant phosphate (Pi) status and the transcription factor PHOSPHATE STARVATION RESPONSE 2 (OsPHR2) are involved in the transcriptional regulation of OsMGD3. CRISPR/Cas9 knockout and overexpression lines of OsMGD3 were generated to explore its potential role in rice adaptation to Pi deficiency. Compared with the wild type, OsMGD3 knockout lines displayed a reduced Pi acquisition and utilization while overexpression lines showed an enhancement of the same. Further, OsMGD3 showed a predominant role in roots, altering lateral root growth. Our comprehensive lipidomic analysis revealed a role of OsMGD3 in membrane lipid remodelling, in addition to a role in regulating diacylglycerol and phosphatidic acid contents that affected the expression of Pi transporters. Our study highlights the role of OsMGD3 in affecting both internal P utilization and P acquisition in rice. (© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
Databáze:	MEDLINE
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