Uncoupling bacterial attachment on and detachment from polydimethylsiloxane surfaces through empirical and simulation studies.

Autor: Pan F; Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland., Liu M; Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland; Department of Earth- and Environmental Sciences, Ludwig Maximilian University of Munich, Theresienstrasse 41, 80333 Munich, Germany., Altenried S; Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland., Lei M; College of Textiles, Donghua University, North Renmin Road 2999, 201620 Shanghai, China., Yang J; Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, CAS, 130022 Changchun, China., Straub H; Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland., Schmahl WW; Department of Earth- and Environmental Sciences, Ludwig Maximilian University of Munich, Theresienstrasse 41, 80333 Munich, Germany., Maniura-Weber K; Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland., Guillaume-Gentil O; Institute of Microbiology, Department of Biology, ETH Zurich, Vladimir-Prelog-Weg 4, 8093 Zurich, Switzerland., Ren Q; Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science and Technology, Lerchenfeldstrasse 5, 9014 St. Gallen, Switzerland. Electronic address: Qun.Ren@empa.ch.
Jazyk: angličtina
Zdroj: Journal of colloid and interface science [J Colloid Interface Sci] 2022 Sep 15; Vol. 622, pp. 419-430. Date of Electronic Publication: 2022 Apr 16.
DOI: 10.1016/j.jcis.2022.04.084
Abstrakt: Bacterial infections related to medical devices can cause severe problems, whose solution requires in-depth understanding of the interactions between bacteria and surfaces. This work investigates the influence of surface physicochemistry on bacterial attachment and detachment under flow through both empirical and simulation studies. We employed polydimethylsiloxane (PDMS) substrates having different degrees of crosslinking as the model material and the extended Derjaguin - Landau - Verwey - Overbeek model as the simulation method. Experimentally, the different PDMS materials led to similar numbers of attached bacteria, which can be rationalized by the identical energy barriers simulated between bacteria and the different materials. However, different numbers of residual bacteria after detachment were observed, which was suggested by simulation that the detachment process is determined by the interfacial physicochemistry rather than the mechanical property of a material. This finding is further supported by analyzing the bacteria detachment from PDMS substrates from which non-crosslinked polymer chains had been removed: similar numbers of residual bacteria were found on the extracted PDMS substrates. The knowledge gained in this work can facilitate the projection of bacterial colonization on a given surface.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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