Use of buccal fat pad-derived stem cells cultured on bioceramics for repair of critical-sized mandibular defects in healthy and osteoporotic rats.

Autor: Camacho-Alonso F; Department of Oral Surgery, University of Murcia, Murcia, Spain. fcamacho@um.es.; Oral Surgery Teaching Unit, University Dental Clinic, Morales Meseguer Hospital (2Nd Floor), Marqués de los Vélez s/n, 30008, Murcia, Spain. fcamacho@um.es., Tudela-Mulero MR; Department of Oral Surgery, University of Murcia, Murcia, Spain., Navarro JA; Department of Histology and Pathological Anatomy, University of Murcia, Murcia, Spain., Buendía AJ; Department of Histology and Pathological Anatomy, University of Murcia, Murcia, Spain., Mercado-Díaz AM; Private Dental Practice, Murcia, Spain.
Jazyk: angličtina
Zdroj: Clinical oral investigations [Clin Oral Investig] 2022 Aug; Vol. 26 (8), pp. 5389-5408. Date of Electronic Publication: 2022 May 07.
DOI: 10.1007/s00784-022-04506-w
Abstrakt: Objective: To compare new bone formation in mandibular symphysis critical-sized bone defects (CSBDs) in healthy and osteoporotic rats filled with bioceramics (BCs) with or without buccal fat pad mesenchymal stem cells (BFPSCs).
Materials and Methods: Thirty-two adult female Sprague-Dawley rats were randomized to two groups (n = 16 per group): group 1 healthy and group 2 osteoporotic (with bilateral ovariectomy). The central portion of the rat mandibular symphysis was used as a physiological CSBD. In each group, eight defects were filled with BC (hydroxyapatite 60% and β-tricalcium phosphate 40%) alone and eight with BFPSCs cultured on BC. The animals were sacrificed at 4 and 8 weeks, and the mandibles were processed for micro-computed tomography to analyze radiological union and bone mineral density (BMD); histological analysis of the bone union; and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2).
Results: In both groups, CSBDs filled with BC + BFPSCs showed greater radiological bone union, BMD and histological bone union, and more VEGF and BMP-2 positivity, compared with CSBDs treated with BC alone at 4 and 8 weeks.
Conclusions: The application of BFPSCs cultured on BCs improves bone regeneration in CSBDs compared with BCs alone in healthy and osteoporotic rats.
Clinical Relevance: Our results may aid bone regeneration of maxillofacial CSBDs of both healthy and osteoporotic patients, but further studies are necessary.
(© 2022. The Author(s).)
Databáze: MEDLINE
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