Differences in Exudates Between Strains of Chlorella sorokiniana Affect the Interaction with the Microalga Growth-Promoting Bacteria Azospirillum brasilense : Differences in Exudates Between Strains of Chlorella sorokiniana Affect the Interaction with the Microalga Growth-Promoting Bacteria Azospirillum brasilense.

Autor: Palacios OA; Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Nuevo Circuito Universitario S/N, Chihuahua, México.; The Bashan Institute of Science, 1730 Post Oak Court, Auburn, AL, 36830, USA., Espinoza-Hicks JC; Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Nuevo Circuito Universitario S/N, Chihuahua, México.; The Bashan Institute of Science, 1730 Post Oak Court, Auburn, AL, 36830, USA., Camacho-Dávila AA; Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Nuevo Circuito Universitario S/N, Chihuahua, México., López BR; The Bashan Institute of Science, 1730 Post Oak Court, Auburn, AL, 36830, USA.; Environmental Microbiology Group, Northwestern Center for Biological Research (CIBNOR), Av. IPN 195, 23096, La Paz, B.C.S, Mexico., de-Bashan LE; The Bashan Institute of Science, 1730 Post Oak Court, Auburn, AL, 36830, USA. luz@bashanfoundation.org.; Environmental Microbiology Group, Northwestern Center for Biological Research (CIBNOR), Av. IPN 195, 23096, La Paz, B.C.S, Mexico. luz@bashanfoundation.org.; Dept. of Entomology and Plant Pathology, Auburn University, 301 Funchess Hall, Auburn, AL, 36849, USA. luz@bashanfoundation.org.
Jazyk: angličtina
Zdroj: Microbial ecology [Microb Ecol] 2023 May; Vol. 85 (4), pp. 1412-1422. Date of Electronic Publication: 2022 May 07.
DOI: 10.1007/s00248-022-02026-4
Abstrakt: The microalga Chlorella sorokiniana and the microalgae growth-promoting bacteria (MGPB) Azospirillum brasilense have a mutualistic interaction that can begin within the first hours of co-incubation; however, the metabolites participating in this initial interaction are not yet identified. Nuclear magnetic resonance (NMR) was used in the present study to characterize the metabolites exuded by two strains of C. sorokiniana (UTEX 2714 and UTEX 2805) and A. brasilense Cd when grown together in an oligotrophic medium. Lactate and myo-inositol were identified as carbon metabolites exuded by the two strains of C. sorokiniana; however, only the UTEX 2714 strain exuded glycerol as the main carbon compound. In turn, A. brasilense exuded uracil when grown on the exudates of either microalga, and both microalga strains were able to utilize uracil as a nitrogen source. Interestingly, although the total carbohydrate content was higher in exudates from C. sorokiniana UTEX 2805 than from C. sorokiniana UTEX 2714, the growth of A. brasilense was greater in the exudates from the UTEX 2714 strain. These results highlight the fact that in the exuded carbon compounds differ between strains of the same species of microalgae and suggest that the type, rather than the quantity, of carbon source is more important for sustaining the growth of the partner bacteria.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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