| Autor: |
Wang D; Yunnan Scientific Observation Station for Cultivated Land Conservation of Ministry of Agriculture and Rural Affairs, Yunnan Agricultural University, Kunming 650201, China.; College of Resources and Environmental Science, Yunnan Agricultural University, Kunming 650201, China., Yi WB; Yunnan Scientific Observation Station for Cultivated Land Conservation of Ministry of Agriculture and Rural Affairs, Yunnan Agricultural University, Kunming 650201, China.; College of Resources and Environmental Science, Yunnan Agricultural University, Kunming 650201, China., Li H; Yunnan Scientific Observation Station for Cultivated Land Conservation of Ministry of Agriculture and Rural Affairs, Yunnan Agricultural University, Kunming 650201, China.; College of Resources and Environmental Science, Yunnan Agricultural University, Kunming 650201, China., Chen LK; Yunnan Scientific Observation Station for Cultivated Land Conservation of Ministry of Agriculture and Rural Affairs, Yunnan Agricultural University, Kunming 650201, China.; College of Resources and Environmental Science, Yunnan Agricultural University, Kunming 650201, China., Zhao P; Yunnan Scientific Observation Station for Cultivated Land Conservation of Ministry of Agriculture and Rural Affairs, Yunnan Agricultural University, Kunming 650201, China., Long GQ; Yunnan Scientific Observation Station for Cultivated Land Conservation of Ministry of Agriculture and Rural Affairs, Yunnan Agricultural University, Kunming 650201, China. |
| Abstrakt: |
Soil microorganism is an important indicator of soil health and plays a critical role in biogeochemical processes. We collected soil samples from a multi-year in-situ field experiment with two cropping modes (maize monocropping and maize-potato intercropping) subjected to four nitrogen (N) levels (N 0 , 0 kg·hm -2 ; N 125 , 125 kg·hm -2 ; N 250 , 250 kg·hm -2 ; N 375 , 375 kg·hm -2 ). By using the Biolog-ECO microplate method, soil microbial metabolic activity, diversity and utilization of six carbon groups were analyzed. The results showed that N application significantly increased the average well color development (AWCD) values, Simpson and Shannon indices, with the highest value at N 250 in mono- and inter-cropped soils. Moreover, N application promoted the utilization of amino acids, amines and phenolic compounds, but decreased the utilization of polymers. Compared with monocropping at the same N level, intercropping improved the AWCD values, Simpson and Shannon indices, with a maximal improvement at N 250 . Intercropping and nitrogen application significantly affected the metabolic activities of the six carbon-source groups. In addition, intercropping improved the utilization of labile carbon sources, such as amino acids and carbohydrates. Results of the redundancy analysis and linear regression showed that intercropping and N application elevated AWCD values by increasing soil temperature, water content, and soil organic carbon content. Therefore, the changes in soil physicochemical properties after intercropping were the main reason for the enhancement of microbial metabolic activity under N application. |
