Biomanufacturing human tissues via organ building blocks.

Autor:	Wolf KJ; Wyss Institute for Biologically Inspired Engineering & John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA 02138, USA., Weiss JD; Department of Bioengineering, Stanford University, 240 Pasteur Drive, Stanford, CA 94304, USA., Uzel SGM; Wyss Institute for Biologically Inspired Engineering & John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA 02138, USA., Skylar-Scott MA; Department of Bioengineering, Stanford University, 240 Pasteur Drive, Stanford, CA 94304, USA; BASE Initiative, Betty Irene Moore Children's Heart Center, Lucile Packard Children's Hospital, Stanford University School of Medicine, Stanford, CA 94304, USA. Electronic address: skyscott@stanford.edu., Lewis JA; Wyss Institute for Biologically Inspired Engineering & John A. Paulson School of Engineering and Applied Sciences, Harvard University, 29 Oxford Street, Cambridge, MA 02138, USA. Electronic address: jalewis@seas.harvard.edu.
Jazyk:	angličtina
Zdroj:	Cell stem cell [Cell Stem Cell] 2022 May 05; Vol. 29 (5), pp. 667-677.
DOI:	10.1016/j.stem.2022.04.012
Abstrakt:	The construction of human organs on demand remains a tantalizing vision to solve the organ donor shortage. Yet, engineering tissues that recapitulate the cellular and architectural complexity of native organs is a grand challenge. The use of organ building blocks (OBBs) composed of multicellular spheroids, organoids, and assembloids offers an important pathway for creating organ-specific tissues with the desired cellular-to-tissue-level organization. Here, we review the differentiation, maturation, and 3D assembly of OBBs into functional human tissues and, ultimately, organs for therapeutic repair and replacement. We also highlight future challenges and areas of opportunity for this nascent field. Competing Interests: Declaration of interests J.A.L. is a cofounder of AcousticaBio and serves as a scientific advisor for Autodesk, Azul3D, Desktop Health (a subsidiary of Desktop Metal), Mooji Meats, and Trestle Biotherapeutics. M.A.S.-S. owns stock in Formlabs, consults for 3D Systems, and is a scientific advisor for AcousticaBio and Mooji Meats. M.A.S.-S., S.G.M.U., and J.A.L. are inventors of the SWIFT biomanufacturing method and have filed a patent on this work. M.A.S.-S. and J.A.L. are inventors on a broader set of patents focused on vascularized human organoids and tissues. Trestle Biotherapeutics has licensed these patents from Harvard University. (Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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