| Autor: |
Pawnikar S; Center for Computational Biology, University of Kansas, Lawrence, KS 66047., Magenheimer BS; Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160.; The Jared Grantham Kidney Institute, University of Kansas Medical Center, Kansas City, KS 66160., Munoz EN; Clinical Laboratory Sciences, University of Kansas Medical Center, Kansas City, KS 66160., Maser RL; Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS 66160.; The Jared Grantham Kidney Institute, University of Kansas Medical Center, Kansas City, KS 66160.; Clinical Laboratory Sciences, University of Kansas Medical Center, Kansas City, KS 66160., Miao Y; Center for Computational Biology, University of Kansas, Lawrence, KS 66047.; Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66047. |
| Abstrakt: |
Polycystin-1 (PC1) is an important unusual G protein-coupled receptor (GPCR) with 11 transmembrane domains, and its mutations account for 85% of cases of autosomal dominant polycystic kidney disease (ADPKD). PC1 shares multiple characteristics with Adhesion GPCRs. These include a GPCR proteolysis site that autocatalytically divides these proteins into extracellular, N-terminal, and membrane-embedded, C-terminal fragments (CTF), and a tethered agonist (TA) within the N-terminal stalk of the CTF that is suggested to activate signaling. However, the mechanism by which a TA can activate PC1 is not known. Here, we have combined functional cellular signaling experiments of PC1 CTF expression constructs encoding wild type, stalkless, and three different ADPKD stalk variants with all-atom Gaussian accelerated molecular dynamics (GaMD) simulations to investigate TA-mediated signaling activation. Correlations of residue motions and free-energy profiles calculated from the GaMD simulations correlated with the differential signaling abilities of wild type and stalk variants of PC1 CTF. They suggested an allosteric mechanism involving residue interactions connecting the stalk, Tetragonal Opening for Polycystins (TOP) domain, and putative pore loop in TA-mediated activation of PC1 CTF. Key interacting residues such as N3074–S3585 and R3848–E4078 predicted from the GaMD simulations were validated by mutagenesis experiments. Together, these complementary analyses have provided insights into a TA-mediated activation mechanism of PC1 CTF signaling, which will be important for future rational drug design targeting PC1. |
