RNase E and HupB dynamics foster mycobacterial cell homeostasis and fitness.

Autor:	Griego A; Institut Pasteur, Université de Paris, Microbial Individuality and Infection Group, F-75015 Paris, France., Douché T; Institut Pasteur, CNRS USR 2000, Mass Spectrometry for Biology Unit, F-75015 Paris, France., Gianetto QG; Institut Pasteur, CNRS USR 2000, Mass Spectrometry for Biology Unit, F-75015 Paris, France.; Institut Pasteur, CNRS USR 3756, Bioinformatics and Biostatistics HUB, F-75015 Paris, France., Matondo M; Institut Pasteur, CNRS USR 2000, Mass Spectrometry for Biology Unit, F-75015 Paris, France., Manina G; Institut Pasteur, Université de Paris, Microbial Individuality and Infection Group, F-75015 Paris, France.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2022 Apr 12; Vol. 25 (5), pp. 104233. Date of Electronic Publication: 2022 Apr 12 (Print Publication: 2022).
DOI:	10.1016/j.isci.2022.104233
Abstrakt:	RNA turnover is a primary source of gene expression variation, in turn promoting cellular adaptation. Mycobacteria leverage reversible mRNA stabilization to endure hostile conditions. Although RNase E is essential for RNA turnover in several species, its role in mycobacterial single-cell physiology and functional phenotypic diversification remains unexplored. Here, by integrating live-single-cell and quantitative-mass-spectrometry approaches, we show that RNase E forms dynamic foci, which are associated with cellular homeostasis and fate, and we discover a versatile molecular interactome. We show a likely interaction between RNase E and the nucleoid-associated protein HupB, which is particularly pronounced during drug treatment and infection, where phenotypic diversity increases. Disruption of RNase E expression affects HupB levels, impairing Mycobacterium tuberculosis growth homeostasis during treatment, intracellular replication, and host spread. Our work lays the foundation for targeting the RNase E and its partner HupB, aiming to undermine M. tuberculosis cellular balance, diversification capacity, and persistence. Competing Interests: The authors declare no competing interests. (© 2022 The Author(s).)
Databáze:	MEDLINE
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