Lysine-based dendrimer with double arginine residues.

Autor: Sheveleva NN; St. Petersburg State University 7/9 Universitetskaya Nab. St. Petersburg 199034 Russia n.n.sheveleva@spbu.ru., Markelov DA; St. Petersburg State University 7/9 Universitetskaya Nab. St. Petersburg 199034 Russia n.n.sheveleva@spbu.ru., Vovk MA; St. Petersburg State University 7/9 Universitetskaya Nab. St. Petersburg 199034 Russia n.n.sheveleva@spbu.ru., Mikhailova ME; St. Petersburg State University 7/9 Universitetskaya Nab. St. Petersburg 199034 Russia n.n.sheveleva@spbu.ru., Tarasenko II; Institute of Macromolecular Compounds, Russian Academy of Sciences Bolshoi Prospect 31, V.O. St. Petersburg 199004 Russia itarasenko@list.ru., Tolstoy PM; St. Petersburg State University 7/9 Universitetskaya Nab. St. Petersburg 199034 Russia n.n.sheveleva@spbu.ru., Neelov IM; St. Petersburg National Research University of Information Technologies, Mechanics and Optics (ITMO University) Kronverkskiy Pr. 49 St. Petersburg 197101 Russia i.neelov@mail.ru., Lähderanta E; Department of Physics, LUT University Box 20 53851 Lappeenranta Finland erkki.lahderanta@lut.fi.
Jazyk: angličtina
Zdroj: RSC advances [RSC Adv] 2019 Jun 07; Vol. 9 (31), pp. 18018-18026. Date of Electronic Publication: 2019 Jun 07 (Print Publication: 2019).
DOI: 10.1039/c9ra02461a
Abstrakt: Due to their well-defined structure, multivalency, biocompatibility, and low toxicity, lysine dendrimers can be used as safe and efficient nanocarriers for drug and gene delivery. One useful strategy for improving the gene delivery properties of dendrimers is modification with arginine amino acid (Arg) residues. Incorporation of Arg residues could be favorable for the enhancement in transfection efficiency of lysine based dendrimers. In this work, we have synthesized a new second-generation poly-l-lysine dendrimer with repeating units containing two linear Arg residues between neighboring lysine branching points (Lys-2Arg dendrimer) and studied its physicochemical properties. We confirmed the structure of Lys-2Arg dendrimer using various one- and two-dimensional 1 H and 13 C NMR spectroscopy methods. Comparison of T 1H relaxation data for Lys-2Arg and Lys-2Lys dendrimers showed that the replacement of double Lys residues with double Arg residues resulted in a sharp decrease in the mobility of methylene groups in side segments and in the main chain of ε-Lys inner segments. We suggest that this unexpected effect is caused by a guanidine-guanidine pairing effect in water, which leads to entanglements between dendrimer branches.
Competing Interests: There are no conflicts to declare.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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