Boron Schiff bases derived from α-amino acids as nucleoli/cytoplasm cell-staining fluorescent probes in vitro .

Autor:	Lara-Cerón JA; Universidad Autónoma de Nuevo León, Facultad de Ciencias Químicas, Ciudad Universitaria Av. Universidad s/n. C. P. 66451 Nuevo León Mexico victor.jimenezpr@uanl.edu.mx +52 81 83760570 +52 81 83294000 ext. 3401., Jiménez Pérez VM; Universidad Autónoma de Nuevo León, Facultad de Ciencias Químicas, Ciudad Universitaria Av. Universidad s/n. C. P. 66451 Nuevo León Mexico victor.jimenezpr@uanl.edu.mx +52 81 83760570 +52 81 83294000 ext. 3401., Xochicale-Santana L; Universidad Autónoma de Nuevo León, Facultad de Ciencias Químicas, Ciudad Universitaria Av. Universidad s/n. C. P. 66451 Nuevo León Mexico victor.jimenezpr@uanl.edu.mx +52 81 83760570 +52 81 83294000 ext. 3401., Ochoa ME; Departamento de Química, Centro de Investigación y de Estudios Avanzados del IPN A.P. 14-740 C.P. 07000 D.F. Mexico., Chávez-Reyes A; Centro de Investigación y de Estudios Avanzados del IPN Unidad Monterrey, PIIT C.P, 66600 Apodaca Nuevo León Mexico.; Escuela de Medicina, Facultad de Medicina, Universidad Finis Terrae Santiago de Chile Chile., Muñoz-Flores BM; Universidad Autónoma de Nuevo León, Facultad de Ciencias Químicas, Ciudad Universitaria Av. Universidad s/n. C. P. 66451 Nuevo León Mexico victor.jimenezpr@uanl.edu.mx +52 81 83760570 +52 81 83294000 ext. 3401.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2020 Aug 26; Vol. 10 (53), pp. 31748-31757. Date of Electronic Publication: 2020 Aug 26 (Print Publication: 2020).
DOI:	10.1039/d0ra05948j
Abstrakt:	The size, shape, and number of nucleoli in a cell's nucleus might help to distinguish a malignant from a benign tumor. Cellular biology and histopathology often require better visualization to understand nucleoli-related processes, thus organelle-specific fluorescent markers are needed. Here, we report the design, synthesis, and fully chemo-photophysical characterization of fluorescent boron Schiff bases (BOSCHIBAs), derived from α-amino acids ( i.e. , phenylalanine, tyrosine and tryptophan), with nucleoli- and cytoplasm-specific staining in cells. It is the first time that Boron Schiff bases derived from α-amino acids act as notorious dual (nucleoli and cytoplasm) cell-staining fluorescent probes. The boron derivatives not only showed good photostability and acceptable quantum yields (∼5%) in solution, but also exhibited low cytotoxicity (>90% cell viability at 0.1 and 1 μg mL -1 ), which make them good candidates to be used in medical diagnosis. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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